《原生质体分离试验方法ppt课件.ppt》由会员分享，可在线阅读，更多相关《原生质体分离试验方法ppt课件.ppt（9页珍藏版）》请在三一办公上搜索。
1、A highly efficient rice green tissue protoplast system for transient gene expression and studying light/chloroplast-related processes,水稻原生质体分离与GFP，BiFC等方法,研究意义,植物原生质体是常见的与生理相关的细胞组织，可用于分析基因的功能特性，有重要意义。在水稻研究中，有关悬浮培养细胞和黄化苗原生质体研究较多，但绿色组织原生质体研究较少。绿色植物原生质体成功用于植物与激素、代谢物及环境因素相关的信号转导的研究。简化并提高了基因短暂表达的系统，并结合蛋白
2、质免疫印染、点位及蛋白质-蛋白质相关试验。,主要内容,Here, we report a simplified method for isolating protoplasts from normally cultivated young rice green tissue without the need for unnecessary chemicals and a vacuum device.,主要内容,Transfections of the generated protoplasts with plasmids of a wide range of sizes (4.5-13 kb)
3、 and co-transfections with multiple plasmids achieved impressively high efficiencies and allowed evaluations by 1) protein immunoblotting analysis, 2) subcellular localization assays, 3) protein-protein interaction analysis.,图片,GFP Chl Merged Bright,GFP Chl Merged Bright,问题7页：The upper panel contain
4、s low power imagesshowing high co-expression efficiency peroxisomes过氧化物酶体,研究结果,Importantly, the rice green tissue protoplasts were photosynthetically active and sensitive to the retrograde plastid signaling inducer norflurazon (NF). Transient expression of the GFP-tagged light-related transcription factor OsGLK1 markedly upregulated transcript levels of the endogeneous photosynthetic genes OsLhcb1, OsLhcp, GADPH and RbcS, which were reduced to some extent by NF treatment in the rice green tissue protoplasts.,
