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1、第4章 生物信息学应用软件,4.1 DNAStar4.2 DNAMAN4.3 GeneDoc,4.1 DNAStar,DNAStar的主要功能：EditSeq 序列编辑GeneQuest 基因搜索MapDraw 图谱绘制MegAlign 序列比对PrimerSelect 引物设计Protean 蛋白分析SeqMan II 序列组装,安装DNAStar应用软件,从“Bioinformatics Programs”文件夹中找Dnastar可执行文件，双击启动安装，安装完成后从“开始” “程序”中点击“DNAstar”，选择所需程序运行。,4.1.1 EditSeq,开始 程序 DNAStar Ed
2、itSeq,Example 4-1,试对下面的DNA序列进行EditSeq分析.ggcacgaggcgacgaaacaatacttgcctgatttaaacgattataagttttgttaaggaaaaactgtgatcagcttcaaattattttcaaattccgcgtaatctccaagatgtcacacaatggaactttggatgaacattatcagaccgtgagggaattttacgatggtaaatccgtttttattacaggagcgaccggattccttggaaaggcgtacgttgagaaacttgcgtattcgtgtccgggcatagtaagcattt
3、acattttgattcgagacaagaaagggtcgaacactgaagagcgaatgagaaaatatcttgatcagcccattttttcacgaataaaatacgaacatccagaatatttcaaaaaaatcattcccatctctggtgatatcaccgcacccaaactaggattgtgcgatgaagaaagaaatatattaatcaacgaggtgtcgatagtgattcactcagccgcttcggtcaaactaaacgatcatttaaaatttactctgaacacgaatgttggaggtacgatgaaagtgttggaactcgttaaag
4、aaatgaaaaacttagcgatgttcgtatatgtgtctacagcttattctaacacaagtcaaagaattttagaagaaaaactttatcctcagtccctaaatctaaacgaaatacagaagttcgctgaagaacattatatattaggtaaagacaacgatgaaatgataaaatttataggtaaccacccaaacacatatgcatacacaaaagcgctggcagaaaacttagttgctgaagaacacggtgaaataccaacaattatcattaggccttcgattattacagcgagtgctgaagagccag
5、tgcgagggttcgtggatagttggagtggtgcaactgctatggcagcatttgctttgaagggatggaacaacataatgtacagcacaggcgaggagaatattgatctgatacctctagactatgttgtcaaccttacattagtagcgattgctaaatataagcctaccaaagaggtcacagtatatcacgtcacaaccagcgatcttaatcccatttcaatccgaagaatatttataaagctctccgaattcgcatcaaaaaatccaacgagtaatgccgcaccattcgcagcaacgactt
6、tattgactaaacagaagccattaataaaactggtcacgtttctcatgcagaccacgccggctttcctagctgacttatggatgaaaacccagaggaaagaagccaaattcgtcaagcaacataatctagtagtgagaagtcgtgatcaactggaattcttcacgtctcagtcgtggttgcttcgttgcgagcgagcccgtgtgcttagtgcggcactgagcgattcagaccgcgctgtcttccgctgcgatccttccaccatagactgggaccaatatttgccaatatacttcgaaggaa
7、tcaacaagcatctgttcaaaaataaattatagtcgattcctaataggcttgtaattgattccaaaaatacttagataacaataactttaatcaactacaatgaaataaatgtaaagtcgtcgtgacctataggataagacgtacggtgcattcgtatatagcgatgcaccggtgttcgaatcccgcaggcggataccaatttttctaatgaaatacgtacttaacaaatgttcacgattgacttccgcggtgaaggaacaacatcgtgtaataaaaatcaaacccgcaaaattataattt
8、gcgtaattactggtggtaggacctcttgtgagtccgcacgggtaggtaccaccactccgtctatttctgccctgaagcagcattgcgtttcggtttgaaaggtggggtagccgtggtaactatactatactatagtaattccaaaattcgctgatttatgttctattagtctgatttagttaaaccatttctatagttactatagttattacctttcttgtactgcacagagacacggaatgctgttttattttaagttttgagttattatagtttaataaatactgtcatattacataa
9、ttatttatgtgcaagatgtaatggccatttagatgtgatacgtcctttttacaaaaaaaaaacatgctatatgaaaaaaaaaaaaaaaaaaa,Paste sequence,Paste your sequence here.,1）Change font size,2）Change Layout (1/2),Change Layout (2/2),Then click here.,Click here.,The changed layout,3）Change to UPPERCASE,Click here.,Change to uppercase,Cli
10、ck here.,The changed letters,4）Find ORF,将光标放到第1个碱基上,Find ORF,Click here.,The found ORF,5）Translate DNA,Result of “translate DNA”,6）Select nucleotides,4.1.2 PrimerSelect,开始 程序 DNAStar PrimerSelect,Example 4-2,以克隆载体pBR322为模板DNA序列，设计引物用来扩增位于2013-2169位的H-strand Y effector。,1）创建PrimerSelect文件,Click here.
11、,Enter sequence,Click here.,Enter sequence,1) Click here.,2) Click here.,3) Click here.,Block view,Sequence view,Click here.,2）定义引物特点和位置,Click here.,Primer characteristics,使用默认值，点击“OK”。,Primer locations (1/4),Click here.,Primer locations (2/4),Click here.,Select “Upper and lower primer ranges”.,Prim
12、er locations (3/4),Click here.,输入相应数值,Primer locations (4/4),3）寻找引物对,Click here.,List of primers,对引物的评价,Click here.,Alternate products,Alternate products,错配点,4）查看引物的其他信息,再点击这里,先选定第一对引物,Amplification summary,Composition summary (1/2),Click here.,Composition summary (2/2),Primer self dimers (1/2),Click here.,Primer self dimers (2/2),Primer pair dimers (1/2),Click here.,Primer pair dimers (2/2),Primer hairpins (1/2),Click here.,Primer hairpins (2/2),
