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1、Parasitic Pathogen Examination,寄生虫病原检查,Etioligic Diagnosis,Stool, blood, marrow, urine, sputum, humores(体液), secretion and tissue.,Purpose of examination,Examining pathogen, identifying the species of parasite and the degree of infection.,Specimen:,Blood and marrow examination,Examining plasmodium,t
2、he collection of blood time：,blood smear thin smear： thick smear：,stain,Wright stainGiemsa stain,Practice 1,Practicing thin smear, stain and observing Plasmodium berghei.,薄血膜制片 厚血制片,取血与涂片,固定与染色,固定 薄血膜晾干后，甲醇固定 染色 将姬氏染液滴于已固定的薄血膜上，染色半小时（室温），自来水冲洗。血片晾干后镜检。,Blood and marrow examination,Examining microfil
3、aria from blood,Time for blood collectingMethods:,Examining L.donovani from marrow,Aspiration (穿刺检查):,Stool Examination,Collect the stool in a dry, clean, leakproof container. Make sure no urine, water, soil or other material gets in the container. Label the container with the patients name , date a
4、nd time of collection.,Significance: Caution:,Stool Examination,Before the patient taking medicine the stool specimen should be collected.,Fresh stool should be examined, or preserved immediately.,Macrography(肉眼观察） Wet mount examination (Direct smear ) Concentration methods Larva hatching methods,Ho
5、w to find parasites in the feces?,Stool Examination,Wet mount method:,Direct smear with saline Iodine staining method（碘染色法）,Stool Examination,Concentration method:,Flotation method:,Stool Examination,Zinc sulfate centrifugal flotation Saturated brine flotation Sucrose sugar flotation,gravitational s
6、edimentation method自然沉淀法 centrifugal sedimentation method离心沉淀法,Stool Examination,Sedimentation method:,larva hatching method:,miracidium hatching method culture method for hookworm larva,How to confirm the density of parasite or worm burden?,Modified Kato-Katzs method Stolls eggs counting,Practice 2
7、,Self-examining stool yourself! Direct smear with saline Saturated brine flotation,Examining parasite from perianal skin,Parasite eggs and adults of pinworm eggs of Taenia Methods cellophane tape method（透明胶纸法） cotton swab method （棉拭子法）,Sputum, Urine, Vaginal secretion, Duodenal fluid, Humores, CSF-,Biopsy and animal inoculation,Summary,Principles of different methods of parasitic diagnosis. ( you should master them.) The processes of experiments.,Preparing for the next class,Contents: Principles Experiments Medical terms,
