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1、HealthNatural Mineral Water,深圳达能益力饮品有限公司 SHENZHEN DANONE YiLi BERVERAGES Co.,Ltd.,降低臭氧浓度控制溴酸盐 与微生物风险控制探讨 DISCUSS BETWEEN MICROBE RISK CONTROL AND THE BROMATE CONTROL BY REDUCING THE CONCENTRATION OF O3,1、臭氧消毒是水处理工作中常用的消毒方法之 一，对国内水生产企业的产品微生物卫生质量 控制的贡献是公认的。臭氧浓度越高效果越好 1 、The ozone sterilize is one of t
2、he in common use in water treatment, and its contribution to control product microbe quality in water industry is acknowledged .The higher concentration the better control effect .,一、臭氧消毒的利与弊The benefit and harm of ozone sterilize,2、臭氧消毒的化学影响： The chemical influence of the ozone sterilize 臭氧是氧的同素异构体
3、 ，是一种优良的氧化剂，臭氧处理矿泉水发生的化学反应较复杂。Ozone is the isomer of the oxygen,it is a good oxidizer ,but the ozone will bring complicated chemical reaction when use in mineral water sterilize process.,氧化反应的最终产物和水的化学成分、PH值、臭氧浓度、反应时间等因素有关。有些氧化反应产物对人体有害，例如溴酸盐，严重影响矿泉水产品质量安全。臭氧浓度越低氧化反应负产物越少。 The oxidation final result
4、 relates to chemical component of water ,PH,ozone concentration,reaction time.some oxidation results is deleterious, eg: bromate,it will severity effect the products safety .The lower ozone concentration the less oxidation results.,二、解决臭氧消毒的利弊矛盾 how to solve the antinomy of benefit and harm about oz
5、one sterilize,选择合适的臭氧浓度既能满足产品微生物控制 要求，又能最大限度降低氧化反应产物负面影 响，达到双赢是追求矿泉水产品质量的最高境界 The best method is to select appropriate ozone concentration,which can not only control the microorganism effective in product but also reduce the influence of the oxidation results.,矿泉水中臭氧杀灭霉菌效果试验 The effect test of steri
6、lize mould by ozone in mineral water,降低臭氧后对微生物的风险控制The microbial risk control after debase the ozone concentration,试 验 目 的 Test purpose,了解掌握霉菌在矿泉水中的存活情况及产品水中臭氧对霉菌的杀灭效果，为选择合适臭氧浓度提供依据 。To know and master the surviving circs of the mould in mineral water and the effect of sterilize mould by ozone.So as
7、 to select the appropriate concentration of ozone.,试 验 材 料 The test material, 500mL PET瓶、瓶盖 500ml PET bottles and some caps, 培养基： medium 虎红琼脂培养基、察氏琼脂培养基（分离培养霉菌用）； Rose Bengal agar and Czapek Dox agar; 抗臭氧霉菌液体培养基；霉菌大样液体培养基； anti-ozone Mold Liquid Medium and Mold Liquid Medium ;,测 试 菌 株 Test strain, 从
8、生产现场接种分离5株霉菌，经鉴定为： Separate 5 strains mould from the mould get in our plant,it dentify :,结实串孢霉（Hormiscium sp.）；,峡筒串孢霉（Prophytroma sp.）,刀孢霉（Clasterosporium sp.）；,卵形孢霉（Oosporeae sp.）；,青霉（Penicillium sp.）；, 广东微生物研究所提供试验用霉菌5株：The 5 mould test strain provide by Guangdong Institute of microbiology,绳状青霉（Pe
9、nicillium funicidosun）MIG3.104,拟康氏木霉（Trichoderma pseadokoningi）MIG3.142,米曲霉（Aspergillus oryzae）MIG3.29,黑曲霉（Aspergillus niger）MIG3.27,桔青霉（Penicillium citrinum）MIG3.100,试 验 方 法 Test method,用无菌生理盐水将新鲜培养的测试菌株 孢子洗下，计数、等量混合，制成孢 子混悬液（12个/ml），备用于定量接种. Wash the new incubated test spore with sterilize 0.9% Na
10、Cl. Count it and mix with same volume ,then execute spore liquid, 制备霉菌孢子混悬液： Prepare turbid solution of the mould spore :,分别调节灌装臭氧浓度至3个目标浓度0.1、0.3、 0.6mg/L，用PET瓶灌装含臭氧矿泉水，立即加 入一系列不同数量0、2.4、4.8、12、36个/500mL 的霉菌孢子，旋盖摇匀。制备样品共计1050个 。 Adjust ozone concentration to 0.1,0.3,0.6 mg/l, filling the ozone wate
11、r to the PET bottle , put the mould spore in it at the same time,and make the quantity to 0; 2.4; 4.8; 12; 36 CFU/500ml, cap it and shake up.The total sample is 1050 bottles.,样品制备： Prepare sample:,灌装完毕即抽样检测作为对照、放置不同时间1、15、 30、60天，半年、1年，每个处理分别抽样10瓶，用霉菌液体大样法（样品摇匀，倒去400ml，瓶中剩100ml，加入三料霉菌液体培养基50ml，30培养4
12、8-72小时）检测瓶中霉菌存活情况。若检出霉菌，以存活阳性率表示。Test the sample at T=0;T=1,15,30,60days and T=6 months and 12months ; test 10 bottles for each concentration and each mould quantity ,add 50ml three times quantity Mold Liquid Medium to 100ml samples,incubate 48-72 hours at 30 ,observe the mould growth .If it have mo
13、uld growth in bottle,it means masculine ., 抽样、检测方法： Sampling and test method,表1：不同臭氧浓度、不同添加菌量、不同时 间灭霉菌效果检测结果 Table 1:The result of different ozone concentration ,different mould spore quantity and different sterilize time., 检 测 结 果 Test result, 检 测 结 果 分 析 Analyse the test result,图1：不同臭氧浓度、添加菌量与阳性率关
14、系图 Chart 1: relatively chart of the masculine and different ozone concentration and different mould spore quantity,根据不同臭氧浓度、不同添加菌量作趋势比较图：Relatively uptrend chart of different ozone concentration and different mould spore quantity.,根据不同臭氧浓度、不同保存时间作趋势比较图：,图2：不同臭氧浓度、保存时间与阳性率关系示意图 Chart 2: relatively ch
15、art of the masculine and different ozoneconcentration and different test time,、成品矿泉水中臭氧浓度在0.1mg/L时霉菌阳 性率高，臭氧浓度在0.3、0.6mg/L 时，霉菌阳性 率低，对霉菌的杀灭效果较好，从控制溴酸盐.降 理化风险角度考虑，选择0.3mg/L臭氧为佳。 There all will have mould growth in finished product at 0.1mg/l ozone concentration, but less mould growth in finish produc
16、t at 0.3;0.6mg/l ozone concentration, they both have good mould sterilize effect .we select 0.3mg/l ozone concentration.,矿泉水中臭氧杀灭霉菌试验结果评估 Evaluate the result of the mould sterilize test in mineral water,、在不同臭氧浓度下，添加菌量大于2个/500 mL 的霉菌存活率较高，即必须控制霉菌污染 2个/500mL，否则再高的臭氧浓度也很难消除 The survival rate is high wh
17、en we add more than 2cfu/500ml mould in the product at different ozone concentrations ,so we should control the mould less than 2cfu/500ml ,otherwise the ozone should be unuseful.,、在不同臭氧浓度下，阳性率与存放时间近似无关 The survival rate is less relative with the shelf time at different ozone concentrations,在控制霉菌污染2
18、个/500mL前提下， 选择臭氧浓度0.3mg/L能有效控制微生物风险 It can control microorganism risk effectively ,when the mould less than 2cfu/500ml and the ozone Concentration is 0.3mg/l., 试 验 结 论 The conclusion of the test,三、霉菌污染控制预防措施 The control measure of mould stain,1、水处理工艺过程控制 Process control,2、PET瓶卫生质量控制 The PET bottle c
19、ontrol,3、输送系统卫生质量控制 The conveying system control,4、灌装无菌室卫生质量控制 The filling room control,5、瓶盖卫生质量控制 The cap control,第一步：制定瓶盖到货检验卫生控制标准： Step 1: establish the microorganism standard of the cap,细菌总数8cuf/ml/5个盖； TPC 8cuf/ml/5caps 霉菌8个/100ml/5个盖； mould 8cuf/100ml/5caps 大肠菌群不得检出 no coliform,图3：2004年度外购瓶盖霉
20、菌检测结果统计图表 The statistical of the mould test result of the caps in year 2004,四、 生产试点： online test 2004年8月，以JVA330线为试点进行降低臭氧浓度生产试验。混合塔臭氧浓度由原0.5-0.7 ppm降低到0.3-0.4 ppm。The test began at August 2004 in JVA 330 , we reduced the ozone concentration to 0.3-0.4ppm from 0.5-0.7ppm in the mixture tank .,第二步：瓶盖
21、臭氧消毒，效果良好 Step 2: Cap sterilize effectively by ozone,五、 生产试点结果 Test Evaluation 图4：330线产品质量合格率统计 statistic the eligibility rate of product quality in 330,2004年8月到至今，对试点线的产品质量跟踪显示， 产品质量持续稳定。We have tracked the quality of the test product since Aug 2004 and find it is well stable continuously,在330线试点成功
22、基础上，2005年2月25日在其他5条水线全线推广，经过1个月质量跟踪评估确认产品质量正常，同年4月在全公司进行降低臭氧浓度工艺变更标准作业。 On Feb 25th 2005, we applied the test in other 5 product line according to the succeed test in 330. And in April we reviewed the standardization process about debase the ozone concentration in whole plant after one month track a
23、nd evaluate.,六、 全面推广、标准化作业 Extend entirely and standardization,表2：臭氧浓度工艺参数执行表Chart2:the implement parameter of ozone concentration,七、结束语 Tag,分享降低臭氧浓度对矿泉水微生物风险控制成功经验Share the succeed experience of the microbe risk control in mineral water by debase the ozone concentration,对溴酸盐控制效果的评估正在进行中，我们期待着，希望在下次达能质量会议上有机会与大家分享。The evaluation of the bromate control effect is doing now, and we hope to share the result with everybody next time.,The End!,Thanks!,
