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1、PCAGGSMCS图SalISnaBICMV-EnhancerAmp+beta-chicken actinpromoterApaIpCAGGS4746 bpsqrAISV40 oriBamHIStuIBamHIpolyAPstIHindIIIXbaIMCSEcoRISacIClaINsiIAsp718-KpnISmaISphIXhoINheIBglII ECORI GAATTC XHOI CTCGAG AVAII GGACC pCAGGS质粒图谱及相关信息 图谱库ID：G0609 质粒名称: pCAGGS 载体类型：mammalian 载体抗性： ampR 培养条件：Grow in stand
2、ard E. coli 37C 相关说明：Start of the nucleotide sequence file in the middle of the first EcoRI site downstream from the AG promoter.;This plasmid is useful for highly efficient expression of genes under the control of the chicken b-actin/rabbit b-globin hybrid promoter (AG) and the human CMV-IE enhance
3、r in various mammalian cells.;The AG promoter sequence consists of the chicken b-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit b-globin fragment, consisting of a 3 part of the second intron (inclusive a branch poin
4、t which is required for normal splicing reactions) and a 5 part of the third exon. When cloning a fragment downstream from the lac promoter it may be advisable to use lacIq strains in order to prevent fortuitous expression of a possibly noxious polypeptide.;This plasmid was completely sequenced at L
5、MBP 特征序列区位： SV40_PA_terminator SV40pro_F_primer SV40_origin M13_reverse_primer 2670 - 2801 2574 - 2593 2512 - 2589 2307 - 2289 1734 - 2247 2328 - 2306 2758 - 2777 4659 - 3799 1533 - 802 2371 - 2342 2477 - 2656 3644 - 3025 4659 - 3799 4729 - 4701 rb_glob_PA_terminator M13_pUC_rev_primer EBV_rev_primer Orf0 Orf0 lac_promoter SV40_promoter pBR322_origin Ampicillin AmpR_promoter
