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1、Excitatory effect of oxytocin on uterine smooth muscle,Shi-Hong Zhang（张世红）Dept of P,Experimental pharmacology of reproductive system,Pituitrin(垂体后叶素)Oxytocin(催产素)Ergot alkaloids(麦角生物碱)Prostaglandins(PGs,前列腺素),Uterine stimulants,Pituitrin(垂体后叶素),oxytocin vasopressin,Uterine stimulants,垂体,Oxytocin(OXT
2、,催产素),1.Pharmacological effects:(1)Uterus:Elevating both the frequency and force of uterine contraction,at rhythm similar to natural labor,highly dependent on estrogen level and condition of uterus.(2)Breast:milk ejection.(3)Blood vessel:vasodilation at large dose.,Uterine stimulants,2.Clinical uses
3、:-to expedite delivery and induced labor;-to reduce the incidence and severity of postpartum hemorrhage.,Uterine stimulants,Oxytocin(OXT,催产素),3.Adverse effects:-Intrauterine asphyxia or uterine rupture(overdose)-Allergy4.Contraindications:-hypertonic dysfunction of uterus-abnormality in birth canal-
4、abnormal fetal position,Uterine stimulants,Oxytocin(OXT,催产素),Ergot alkaloids(麦角生物碱)Ergometrine(麦角新碱),Ergotamine(麦角胺),Ergotoxine(麦角毒),1.Pharmacological effects:(1)Uterus(ergometrine):-strongly stimulating uterine smooth muscle,both the body and the cervix;ready to induce tonic contraction at larger d
5、ose.(2)Blood vessel(ergotamine):-contracting peripheral blood vessels and cerebral vessels.(3)blocking receptor(ergotoxine):,Uterine stimulants,2.Clinical uses:(1)Endometrorrhagia(子宫出血),and puerperal replacement of uterus(产后子宫复位):ergometrine;(2)Migraine(偏头痛):ergotamine;(3)Hibernation therapy(冬眠疗法):d
6、ihydroergotoxine+pethidine+promethazine.,A.Uterine stimulants,Ergot alkaloids(麦角生物碱),3.Adverse effects:-nausea,vomiting,BP;-allergic reaction;-prohibited in expediting delivery and induced labor.,A.Uterine stimulants,Ergot alkaloids(麦角生物碱),Prostaglandins(PGs,前列腺素),Dinoprostone(地诺前列酮,PGE2)Dinoprost(地
7、诺前列素,PGF2)1.Pharmacological effect:stimulating uterine smooth muscle.2.Clinical uses:to induce labor2.Adverse effects:GI reaction,attack of asthma(PGF2);glaucoma(PGE2).,Uterine stimulants,Objectives,To observe the excitatory effect of oxytocin on uterine smooth muscles and its dose dependent manner.
8、,Methods,Estrodiol pretreatment(0.2mg)on rats(200 g 40g)24hr before experiment;Prepare uterus smooth muscle specimen.,Methods,Uterus of the rat,Methods,Uterus of the rat,Methods,Perfusion system,Methods,Set parameters of recording system:Input pattern:tensilityGain:200-500Lower frequency:direct curr
9、entUpper frequency:10 HzSampling interval:2 msPreload:1g,Methods,The specimen is stabilized in the perfusion chamber for 15-30 min until the contractive force and rhythm become regular.Record the basal contractive force and frequency(optional).Add 0.02 ml,0.04 ml,0.14 ml oxytocin(0.01U/ml)into the p
10、erfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,Methods,Add 0.04 ml,0.14 ml oxytocin(0.1U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.Add 0.04
11、 ml,0.14 ml oxytocin(1U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each drug concentration accordingly.,Methods,Add 0.04 ml,0.14 ml oxytocin(10U/ml)into the perfusion system.Record the maximal force and frequency(optional)of contraction at each dru
12、g concentration accordingly.,Methods,Notice:1Avoid injury and excessive stretch of uterus during the whole process of preparation.2Control the gas bubbles in a proper rate in case of impact on uterus contraction.3Record a period of baseline of contraction before drug treatment in order to compare that before and after.4Add drug in the order as prescribed.,Lab report,1题目2署名:作者和合作者姓名及单位。3结构式摘要（目的，方法，结果，结论）。4材料和方法:主要实验材料，标本制备方法，仪器装置连接和仪器参数，实验数据记录条件，观察指标。5结果:实验结果的文字性描述，作量效曲线。6讨论:回答思考题。,Learning issue,1缩宫素对子宫的作用及特点。2为什么实验前用雌二醇处理动物？3.缩宫素与麦角新碱对子宫平滑肌的作用特点有何不同？,
