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1、离子色谱的色谱柱技术,重点内容,色谱的分离原理-三种重要的分离方式：离子交换、离子排斥、反相离子对新型阴离子色谱柱-高交换容量柱、氢氧根淋洗液柱、可变交换容量柱新型阳离子色谱柱-接枝型羧酸型阳离子色谱柱其它公司的离子色谱柱-万通、Alltech等判断色谱柱好坏的标准-柱效、分离时间、水负峰、阳离子一价和二价可否同时分离,分离的方式,离子排斥：离解常数 和疏水性 离子对(RP)：对离子对试剂的亲和力 离子对化合物的疏水性离子抑制(RP)：在特定 pH下的疏水性反相：疏水性,离子交换：离子价态和离子半径,Temporal course,离子交换分离机理,阴离子交换树脂(乳胶型）,m,阳离子交换树脂
2、(接枝型),分离阴离子,Column：IonPac AG12A/AS12AEluent：2.7mM Na2CO3 0.3mM NaHCO3Flow rate：1.2mL/minDetection：Conductivity(ASRS Recycle mode),0,2,4,6,8,10,12,14,16,Retention time(min),0,8,mS,Column:IonPac CS12A,CG12AEluent:20 mM Methanesulfonic acidFlow rate:1.0 mL/minDetection:Conductivity（CSRS Recycle mode),分
3、离阳离子,0,2,4,6,8,10,12,14,Retention time(min),0,1,2,3,4,S,离子排斥法分离机理,Donnan membrane,固定相,流动相,离子排斥色谱法的分离机理,1.Donnan排斥作用Donnan膜的负电荷层排斥完全离解的离 子型化合物，仅允许未离解的化合物通过 2.吸附保留时间与有机酸的烷基键的长度有关。通常烷基键越长，其保留时间也越长。3.空间排阻与有机酸的分子量大小及交换树脂的交联度有关,Column：IonPac ICE AS1Eluent：0.4 mM Octonesulfonic acidFlow rate：1.0 mL/minSupp
4、ressor：AMMS-ICERegenerator liquid：5mM TBAOH 50mM H3BO3Detection：Conductivity,离子排斥法分离有机酸,20,10,0,保留时间(min),0,S,5,1,2,3,4,5,1.甲酸2.乙酸3.丙酸4.丁酸5.戊酸,Mobile phase TBAOHACNH2OSampleY+X-,固定相,TBA+OH-,Y+X-,Y+X-,(TBA+X-),(TBA+X-),(TBA+X-),(TBA+X-),TBA+OH-,ACN,ACN,ACN,ACN,ACN,ACN,ACN,TBA+OH-,TBA+OH-,疏水相,亲水 相,反相离
5、子对分离机理,Y+OH-,反相离子对的分离机理,1.生成离子对待测离子与离子对试剂生成中性“离子对”分布于 固定相与流动相之间，其分离类似传统的反相分离2.动态离子交换离子对试剂的疏水部分吸附于固定相形成动 态的离子交换表面，其分离机理类似于离子交换。3.离子间相互作用除包括以上两种分离机理和固定相表面双 电层结构的分离机理。,Column:IonPac NS1、NG1 Eluent:2 mM TBAOH,24%48%CAN gradient in 10 min Flow rate:1.0 mL/min Detection:Conductivity(suppressor type)（ASRS
6、MPIC Mode）Regenerator:10 mN H2SO41.Methanesulfonic acid 5.0 g/mL(ppm)2.1-Propanesulfonic acid 8.63.1-Butanesulfonic acid 8.74.1-Hexanesulfonic acid 8.85.1-Heptanesulfonic acid 8.96.1-Octanesulfonic acid 8.97.1-Decanesulfonic acid 9.1,0,Retention time(min),12,16,8,S,0.0,4,8.0,反相离子对分离测定,不同的分离方式的应用范围,离
7、子交换-对常用，用于阴、阳离子的分离和检测，对不同的分离有不同的选择。离子排斥-主要有弱电离阴离子物质的分离，主要有ICE-AS1，AS5等反相离子对-用于离子表面活性剂和相对亲脂性离子的检测，主要有NS1，PAX500，PCX500,离子交换色谱中固定相的选择,基体性质-多孔性-组成-交联度交换容量亲脂性,IonPac AS4A-SC填料的薄壳结构,6.5 m,高交联核,阴离子交换乳胶,8235-02,16070,交换容量(meq;4 x 250 色谱柱),色谱柱,胶乳附聚微孔基质阴离子交换色谱柱,IonPac AS16色谱柱填料结构,阴离子交换胶乳层,80-nm 直径微孔,乙基乙烯苯-二乙
8、烯苯核,14330,胶乳附聚超大孔基质阴离子交换色谱柱,16071,交换容量(meq;4 x 250色谱柱),色谱柱,AS9-HC,AS10,AS11-HC,AS12A,AS16,190,170,290,52,170,15917,IonPac AS14A 色谱柱填料结构,高交联核,阴离子交换树脂层,3.5 m,16072,交换容量(meq;4 x 250色谱柱),色谱柱,AS14,AS14A,AS15,65,120,225,接枝大孔基质阴离子交换色谱柱,14211A,各类阴离子柱固定相的性质,色谱柱,乳胶或接枝,交换容量(每4 mm色谱柱),功能基,亲脂性,碳酸盐淋洗,氢氧根淋洗,IonPac
9、 AS4A-SC L 20 meq 季铵烷醇 中低IonPac AS9-SC L 30-35 meq 季铵烷 中低IonPac AS9-HC L 190 meq 季铵烷 中低IonPac AS10 L 170 meq 季铵烷醇 低IonPac AS11 L 45 meq 季铵烷醇 极低IonPac AS11-HC L 290 meq 季铵烷醇 中低IonPac AS12A L 52 meq 季铵烷 中等IonPac AS14 G 65 meq 季铵烷 中高IonPac AS15 G 225 meq 季铵烷醇 中高IonPac AS16 L 170 meq 季铵烷醇 超低,高交换容量,PO32-
10、,5 nm,接枝的阳离子交换树脂,8094-05,大孔聚合物基体表面积=450 m2/g,4.5 mm,阳离子交换分离柱的性质,16096,OmniPac PCX-1008.555120磺酸1IonPac CS108.55580磺酸1IonPac CS118.05535磺酸1IonPac CS128.5552800羧酸450IonPac CS12A8.5552800羧酸450磷酸IonPac CS158.5552800羧酸450磷酸冠醚IonPac CS165.5558000羧酸 450,色谱柱颗粒 基体交换容量功能 表面积 直径(mm)(%X-交联)(eq/column)基(m2/g),碳酸
11、盐选择性色谱柱的发展历程,15049,1987,1982,1975,快速阴离子,AS4,Small et al.发表第一篇IC论文,1991,AS4A-SC,1992,AS9-SC,1980,AS1,1981,AS2&AS3,1984,AS4A,1994,AS12A,1997,AS9-HC,AS14A,2000,氢氧化物选择性色谱柱的发展历程,14236-01,1999,1991,1989,1986,1983,1975,AS10,AS5A,AS5,Small et al.发表第一篇IC论文,1992,AS11,1998,AS15&AS16,AS17,1997,AS11-HC,PAX-100&P
12、AX-500,2000,AS15-5m,2002,穴状配体A1,离子色谱测定各类物质的分离和检测总结,新型无试剂离子色谱(RFIC)用的色谱柱和消耗件介绍,IonPac AS18 阴离子交换柱 IonPac CS17 阳离子交换柱EGC II 罐和EG40转换线 CR-TC连续再生捕获得柱,氢氧根选择性阴离子交换柱,AS11 对于快速有机酸和样品扫描的最优色谱柱,凝胶乳液型AS11-HC 高交换容量的有机酸分析适用于高浓度范围,凝胶乳液型AS15 等淋洗柱设计用于电场的应用,节枝聚合物AS16 高容量对极性阴离子最佳色谱柱,凝胶乳液型AS17 低容量型最佳对于梯度淋洗饮用水的分析,凝胶乳液型,
13、IonPac AS18 阴离子交换柱,为什么IonPac AS18柱重要?,氢氧化物选择性柱用于分离无机阴离子扩展无试剂离子色谱操作常规阴离子的分析 通用型操作-等淋洗氢氧根用于常规无机阴离子的分析-氢氧根梯度用于复杂基体的分析高容量(285 equiv/色谱柱)-允许大的进样量色谱柱在30C时有最佳的分离效率-确保重现性的保留时间,乳胶凝合超孔树脂,阴离子交换胶乳涂层,乙基乙烯苯 二乙烯苯核心,关键IonPac AS18色谱柱的应用,目标是IonPac AS4A-SC,AS12A,AS14,AS14A和 AS17无机阴离子的全部应用常规阴离子络合物样品复杂-饮用水-废水-化学处理溶液-工业冷
14、却水-食品和饮料,快速等淋洗无机阴离子分离,色谱柱:IonPac AS18(4 mm)淋洗液:39 mmol/L KOH 淋洗源:EGC-KOH with CR-ATC温度:30C流速:1.0 mL/min进样量:25 L检测器:抑制电导,ASRS-ULTRA(4 mm),150 mA,自动再生,循环模式Peaks:1.氟离子1 mg/L 2.氯离子 23.亚硝酸根54.硫酸根 6 5.溴离子10 6.硝酸根 10 7.磷酸根20,色谱柱:IonPac AS18 4 mm 淋洗液:33 mM 氢氧化钾淋洗源:EGC-KOH with CR-ATC温度:22 C(ambient)流速:1.0 m
15、L/min进样量:25 L检测器:抑制电导,ASRS ULTRA 4 mm,100 mA自动抑制 循环模式色谱峰:1.氟离子0.5mg/L(ppm)2.乙酸根2.53.甲酸根1.04.亚氯酸根55.氯离子 36.碳酸根207.亚硝酸根58.硫酸根109.溴离子1010.硝酸根1011.氯酸根1012.磷酸根10,等淋洗分离无机阴离子在IonPac AS18 Column,0,5,10,15,-2.0,16,Minutes,S,4,3,1,2,5,12,11,10,9,8,7,6,IonPac AS18是否能用于EPA-和ISO方法?,正是U.S.EPA方法300.0 Part(A)需要U.S.
16、EPA批准氢氧根淋洗液用于EPA方法300.0和 300.1正是ISO需要,色谱柱:IonPac AS18(4 mm)淋洗液:KOH:2240 mmol/L at 78 min淋洗源:EGC-KOH with CR-ATC温度:30C流速:1.0 mL/min进样量:100 L检测器:抑制电导,ASRS-ULTRA(4 mm),100 mA,自动抑制,循环模式色谱峰:1.氟离子8.碳酸根 2.乙酸根 9.溴离子 3.未知 10.硫酸根 4.甲酸 11.硝酸根 5.氯离子 12.氯酸根 6.亚硝酸根 13.磷酸根 7.未知,用IonPac AS18柱测定饮用水中的阴离子,色谱柱:IonPac A
17、S18淋洗液:KOH:1244 mmol/L from 05 min and 4452 mmol/L from 810 min淋洗源:EGC-KOH with CR-ATC温度:30C 流速:1 mL/min进样量:25 L检测器:抑制电导,ASRS-ULTRA自动抑制,循环模式色谱峰:1.氟离子0.511.Selenate10 mg/L 2.Acetate2.512.Nitrate10 3.Formate113.Chlorate10 4.Chlorite514.Phosphate 10 5.Chloride315.Molybdate10 6.Nitrite 616.Tungstate 10
18、7.Selenite1017.Arsenate10 8.Sulfite1018.Thiosulfate10 9.Sulfate1019.Chromate10 10.Bromide10,快速双梯度分离不同的无机和有机阴离子,色谱柱:IonPac AS18淋洗液:KOH:1244 mmol/L from 05 min and 4452 mmol/L from 810 min淋洗源:EGC-KOH with CR-ATC温度:30 C流速:1.0 mL/min进样量:100 L检测器:抑制电导,ASRS-ULTRA 自动抑制,循环模式样品准备:1:100 稀释色谱峰:1.Fluoride11.Sul
19、fite 2.Acetate12.Sulfate 3.Formate13.Bromide 4.Unknown14.Unknown 5.Unknown 15.Nitrate 6.Unknown 16.Unknown 7.Unknown 17.Unknown 8.Chloride 18.Thiosulfate 9.Nitrite 19.Unknown10.Carbonate,快速双梯度快速分离废水,IonPac AS18色谱柱介绍,有标准内径和微孔型2003年四月开始出售New AS18 PIBAS18新产品手册AS18出售培训手册其它支持文献-电子目录-e-News Brief-Website
20、info-Press release,IonPac CS16阳离子交换柱,IonPac CS16-5m(5 x 250 mm),流速:1.0 mL/min进样量:25 L温度:40C检测:抑制电导,自动抑制,循环模式色谱峰:1.锂离子0.1 mg/L2.钠离子0.43.氨离子0.54.钾离子1.05.镁离子0.56.钙离子1.0,30 mmol/L MSA,Minutes,0,5,10,15,20,25,0.20,1.60,1,2,3,4,5,6,S,0,5,10,0.29,1.93,1,2,3,5,4,6,S,48 mmol/L MSA,第I 和 II组阳离子用IonPac CS16-5mm
21、(5 x 250 mm),淋洗液:40 mmol/L MSA流速:1 mL/min温度:65C进样量:25 L检测器:抑制电导,自动抑制,循环模式(抑制器在加热之外)色谱峰:1.Lithium 0.1 mg/L2.Sodium 0.43.Ammonium 0.5 4.Potassium 1.0 5.Rubidium 5.0 6.Magnesium 0.5 7.Cesium 5.0 8.Calcium 1.0 9.Strontium 5.0 10.Barium 5.0,极高的浓度比用IonPac CS16-5mm(5 x 250 mm),Column:IonPac CS16-5m and gua
22、rdFlow rate:1 mL/minTemperature:40CInj.volume:25 LDetection:Suppressed conductivity,AutoSuppression,Recycle ModePeaks:1.Sodium 100.00 mg/L2.Ammonium 0.01,30 mmol/L MSA,0,2,4,6,8,10,0.19,0.29,2,S,1,Minutes,0,2,4,6,8,0.32,0.42,2,1,S,48 mmol/L MSA,在大量氨中分析痕量的钠离子,Column:IonPac CG16,CS16-5m(5 x 250 mm)Elu
23、ant:30 mmol/L MSATemperature:40CFlow rate:1 mL/minSample:Industrial wastewater,1:10 dilutionInj.volume:25 LDetection:Suppressed conductivity,AutoSuppression,Recycle ModePeaks:1.Sodium 0.01 mg/L2.Ammonium 100,自来水样品用IonPac CS16-5m(5 x 250 mm),Flow rate:1 mL/minTemperature:40CInj.volume:25 LDetection:S
24、uppressed conductivity,AutoSuppression,Recycle Mode CalculatedPeaks:1.Lithium0.002 mg/L2.Sodium 19.7303.Ammonium0.0654.Potassium0.9875.Magnesium7.2106.Calcium 18.544,IonPac CS17阳离子交换柱,IonPac CS17 Column Features,新型的树脂结构用于亲脂性和多价胺类设计中等交换容量(1.45 mequiv/column)-对于胺类的淋洗有比较适中的酸浓度用简单的甲磺酸梯度对大多数用CS14,CS15,和C
25、S16胺的应用可以不必加有机溶剂来改善峰形简单的无试剂离子色谱的操作,新的阳离子交换固定相的胺的分离示意图,节枝羟酸阳离子聚合层,边界层,55%交联度7 m 大孔型450 m2/g(EVB-DVB)颗粒核,(EVB-DVB)颗粒核,孔的结构,IonPac CS17的主要应用,亲脂性和多价胺生物胺类脂肪胺类双胺类对亲水性胺类包括醇胺类分离比较理想对于安培检测比较理想对于无机阳离子和小分子胺的IC-MS扫描比较理想,色谱柱:IonPac CS17(4 mm)温度:30C淋洗液:A.6 mmol/L MSA淋洗液:B.2 mmol/L MSA isocratic to 11 min;step cha
26、nge to 7 mmol/L MSA at 11.1 min;isocratic to 22 min;step change back to 2 mmol/L MSA at 22.1 min;equilibration time is 2 min流速:1 mL/min进样量:25 L抑制器:Cation Atlas Electrolytic Suppressor,AutoSuppression,Recycle Mode色谱峰:1.Lithium0.1 mg/L 2.Sodium0.43.Ammonium0.54.Potassium1.05.Dimethylamine1.06.Triethyl
27、amine7.07.Magnesium0.58.Calcium1.0,比较等淋洗和梯度在IonPac CS17,用IonPac CS17测定生物胺类,Column:IonPac CG17,CS17(2-mm)Temperature:40CEluant:3 mmol/L MSA isocratic to 3.5 min;3 mmol/L to 6 mmol/L MSA gradient at 12 min;isocratic to 15 min;gradient to 40 mmol/L MSA at 20 min,isocratic to 24 minFlow rate:0.40 mL/min
28、Inj.volume:25 LSuppressor:CSRS-ULTRA 2-mm Cation Self-Regenerating Suppressor,AutoSuppression,Recycle ModePeaks:1.Lithium10g/L2.Sodium403.Ammonium504.Potassium1005.Magnesium506.Calcium1007.Putrescine10008.Cadaverine6009.Histamine60010.Spermidine20011.Spermine400,Column:IonPac CG17,CS17,2 mmTemperatu
29、re:30CEluant:0.5 to 0.7 mmol/L MSA gradient at 25 min;step change to 4 mmol/L MSA at 25.1 min;isocratic to 27 min;step change to 6.5 mmol/L MSA at 27.1 min;isocratic to 37 minFlow rate:0.35 mL/minInj.volume:25 LSuppressor:Cation Atlas Electrolytic Suppressor AutoSuppression,Recycle ModePeaks:1.Lithi
30、um 25 g/L2.Sodium 1003.Ammonium 1254.Hydrazine10005.Ethanolamine 2506.Potassium 2507.2-(2-aminoethoxy)ethanol10008.5-amino-1-pentanol 5009.Morpholine100010.2-diethylamino ethanol100011.Cyclohexylamine100012.Magnesium 12513.Calcium 250,梯度淋洗锅炉用水胺添加剂的分离在IonPac CS17,梯度淋洗分离二胺在IonPac CS17,用IonPac CS17电化学检
31、测,Column:IonPac CS17(4 x 250 mm)Temperature:30C Eluant:1.8 mmol/L MSA isocratic to 8 min;gradient to 6 mmol/L MSA at 11 min;isocratic to 15 min;step change to1.8 mmol/L MSA at 15.1 minFlow rate:1 mL/minDetection:Integrated Pulsed Amperometry Au electrode,AAA waveform 1 mol/L NaOH post-column at 1 mL
32、/min Peaks:1.Hydroxylamine 1 mg/L 2.Methylhydroxylamine 5 3.Ethylhydroxylamine 404.Dimethylhydroxylamine 55.Diethylhydroxylamine 8.5,IC-MS扫描梯度对小分子胺和其它阳离子用 CS17(250 mm x 2 mm),1 mM MSA,10 mM MSA,Extracted ion chromatograms:Sodium,m/z 23Ammonium,m/z 18Hydrazine,m/z 32Potassium,m/z 392-(2-Aminoethoxy)e
33、thanol,m/z 1065-Amino-1-pentanol,m/z 104Morpholine,m/z 882-Diethylaminoethanol,m/z 118Cyclohexylamine,m/z 100Magnesium,m/z 24Calcium,m/z 40,0,2,4,6,8,10,12,14,16,Minutes,11,10,9,8,7,6,5,4,3,2,1,Cond.,IonPac CS17柱的介绍,Available in standardbore and microbore formatsShipments started December 10,2002New
34、 CS17 PIBCS17 Product ManualCS17 Sales Training ManualOther support literature-e-Catalog-e-News Brief-Website info-Press release,EGC II 罐和EG40适配线,EGC II罐和EG40适配线升级,New EGC II Cartridges ship with new Power Connector-New EGC II part numbers have been assigned(058900 and 058902)-EG40 Adaptor cable req
35、uired for EG40 installation(057899)The existing EGC Cartridge part numbers(053921 and 053922)will be discontinued on June 30,2003,CR-TC连续再生捕获柱,什么是CR-TC?,CR-TC连续再生捕获柱从淋洗发生器罐中去除离子态污染而不必离线再生CR-TC代替常规的捕获柱用于淋洗液发生CR-TC与淋洗液发生器的脱气组件相连接,为什么要用CR-TC连续再生捕获柱?,用CR-TC提纯淋洗液使离子色谱得到超纯的色谱效能 整体式改善碳酸或氨的干扰减少色谱峰得到理想的响应值灵敏
36、度改善,CR-ATC,连续再生捕获柱 新概念,CRATC硬件示意图,Pt 正电极(H2O 2H+O2+2e-),阳离子交换连接器,KOH 发生室,孔,泵,H2O,Pt 阴极(2H2O+2e-2OH-+H2),HCO3-阴离子捕获得,+,-,KOH+H2,KOH,H2,K+电解池,K+,脱气单元,EG40氢氧根淋洗发生用于阴离子分析,EluGen KOH 罐,CR-ATC阴离子捕获,X,CR-TC垂直于EG40和EG50,EGDegas,柱,进样阀,池,废水,再生液进,再生液出,至脱气,反压管,绿 管0.03”ID,淋洗液进,淋洗液出,EG50,抑制器,CR-TC,SRS 废液进,泵,GM3/G
37、M4混合器,AS15梯度淋洗应用于EG40,Column:IonPac AS15(4 x 250 mm)Eluant:EG40 generated KOHTime(min)Concentration(mmol/L)01.020.050.025.050.0Flow rate:1.5 mL/minInj.volume:10 LDetection:Suppressed conductivityTemperature:30 CSuppressor:ASRS-ULTRA,4-mm,recycle modeSample:1.Fluoride 0.2 mg/L2.Chloride 0.3 mg/L 3.Ca
38、rbonate NA 4.Sulfate 1.5 mg/L5.Nitrate 1.0 mg/L6.Phosphate 1.5 mg/LBackground(S/cm)StartEndDriftEG400.640.880.24ATC-HC0.410.490.08CR-ATC0.370.430.06,AS18步进梯度应用 停机周未以后重新启动,Column:Prototype IonPac AS18(4 x 250 mm)Eluant:EG40 generated KOHTime(min)Concentration(mmol/L)01512.73017.530Flow rate:1.0 mL/mi
39、nInj.volume:10 LDetection:Suppressed ConductivityTemperature:30 CSuppressor:ASRS-ULTRA,4 mm,recycle modeSample:Peaks:1.Fluoride 0.2 mg/L2.Chloride 0.3 3.Nitrite1.0 4.Carbonate NA 5.Bromide1.0 6.Nitrate1.0 7.Sulfate 1.5 8.Phosphate 1.5,EG CR-ATC,EG ATC-HC,Why is a Lower Background Beneficial for Isoc
40、ratic Applications?Effect of Residual Carbonate on Peak Area Response,Column:AS15(4mm)Eluant:38.0 mM KOH(EG40)Flow rate:1.2 ml/minSuppressor:ASRS-ULTRA(4-mm)Recycle ModeInj.volume:10 LPeak areaSample:DI water10 M KHCO3 mg/LFluoride0.30090.2819 2.0Chloride0.22880.2166 3.0Nitrite0.38860.3591 10.0Sulfa
41、te0.89820.8542 15.0Bromide0.31690.2929 10.0Nitrate0.61500.5700 10.0Phosphate0.33340.3267 15.0with CR-ATCSampleDI water10 M KHCO3 mg/L Fluoride0.34210.3433 2.0Chloride0.27540.2764 3.0Nitrite0.44830.4525 10.0Sulfate1.01811.0281 15.0Bromide0.39550.4004 10.0Nitrate0.75500.7564 10.0Phosphate0.46160.4632
42、15.0,0,2,4,6,8,10,12,14,16,18,20,22,24,26,28,30,0,2.5,5.5,S,Minutes,2,1,0.34 S/cm,EG Control,EG CR-ATC,Why is a Lower Background Beneficial for Gradient Application?Effect of Residual Carbonate on Peak Area Response,Column:AS15(4mm)Eluant:10.0 mM to 45.0 mM KOH in 8 min(EG40)Flow rate:1.6 mL/minSupp
43、ressor:ASRS-ULTRA(4-mm)recycle modeInj.volume:10 LPeak areaSample:DI water10 M KHCO3 mg/L,Fluoride0.24750.2257 2.0Chloride0.19240.1784 3.0Nitrite0.29620.2657 10.0Sulfate0.61700.5054 15.0Bromide0.20690.1575 10.0Nitrate0.41190.3580 10.0Phosphate0.26450.2230 15.0with CR-ATCSampleDI water10 M KHCO3 mg/L
44、Fluoride0.24370.2411 2.0Chloride0.19780.1993 3.0Nitrite0.32730.3270 10.0Sulfate0.65070.6507 15.0Bromide0.25160.2549 10.0Nitrate0.49470.4926 10.0Phosphate0.32080.3242 15.0,EG Control,EG CR-ATC,Removes ionic contaminants from EGC eluantsNo regeneration steps requiredContinuous operation with no down t
45、imeNo user intervention requiredSaves time and moneyFast IC system startup time after shutdownImproved chromatographic performance over premixed eluantsLower baseline driftImproved retention time stabilityImproved peak area reproducibilityImproved detection limitsCarbonate interference is minimized,
46、Continuously Regenerated Trap Columns:Performance Benefits,IonPac CR-TC Introduction,Shipments started February 7,2003Product PIBs-Concentrator and Trap Column PIB-New EG50 PIB-New RFC-10/RFC-30 PIB New CR-TC Product ManualOther Support Literature-Electronic catalog-E-News Brief-Website info,Summary
47、 of RFIC Consumables Introductions,IonPac AS18 Anion Exchange ColumnIonPac CS17 Cation Exchange ColumnEGC II Cartridges and EG40 Adapter CableCR-TC Continuously Regenerated Trap Columns Reference Library CD-Rom Rev.18E-Catalog CD-Rom for March 2003 Edition 01March 2003 System Price Lists,The“Metrohm
48、”Column Technology万通公司的色谱柱,Typical Anion Chromatogram,Separator:Metrosep Anion Dual 1Flow rate:0.5 mL/minEluant:9 mmol/L Phthalic acid-ACN(98:2 v/v),Detection:Non-suppressed conductivitypH 4.2 with TrisInj.volume:50 LPeaks:5 mg/L each of fluoride(1),chloride(2),nitrite(3),bromide(4),nitrate(5),sulfa
49、te(6),iodide(7),thiosulfate(8)and thiocyanate(9),Typical Anion Chromatogram,Separator:Metrosep Anion Dual 2Flow rate:0.5 mL/minEluant:1.3 mmol/L Na2CO3+2 mmol/L NaHCO3Detection:Suppressed conductivity Inj.volume:20 LPeaks:2 mg/L fluoride(1),5 mg/L each of chloride(2)and nitrite(3),10 mg/L each of br
50、omide(4),nitrate(5),orthophosphate(6)and sulfate(7),Typical Anion Chromatogram,Separator:Metrohm SUP 5(Shodex SI-50 4E)Flow rate:0.7 mL/minEluant:3.2 mmol/L Na2CO3+1 mmol/L NaHCO3Detection:Suppressed conductivity Inj.volume:20 LPeaks:2 mg/L fluoride(1),3 mg/L chloride(2),5 mg/L nitrite(3),10 mg/L ea
