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1、针行方便揿针针刺原始效应物质的研究 Study on Acupuncture Original Material,目的 将下述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，共同组成一个完整的证据锁链，证明针刺原始效应物质、针刺电化学效应机制。,Objective We will practice the following Four experiments made by the evidence,electrochemical and corrosion original micro-battery effect scientific laws,the common compos
2、ition of a complete chain of evidence，and will prove that the effect of acupuncture raw materials,electrochemical effects of acupuncture mechanism.,实验1 方便揿针针刺电化学电流研究实验1-1 人体针刺电化学电流研究,Experiment 1-1 Electrochemical current study by acupuncture,方法 将针灸针分别垂直刺入实验对象的肩髃、肩髎穴位中，用数字显示电流仪测量记录产生的电流变化,Methods Ac
3、upuncture needles were stung vertically to alienate experiments of the points of Jianyu,Jian liao,with figures current measurement records showed the changes of the current.,结果 不锈钢针灸针刺入人体穴位，所测的9名对象电流变化在-44 67A区间，电流差值为27 73A。,Result Stainless steel acupuncture were needled into the human body,measuri
4、ng the changes on the current target is-44 67 uA interval,the current margin was 27 73 A on the cave of 9 cases.,表1-1、针刺肩髃、肩髎穴的电流测定记录表（男=m、女=w）Table 1、Statistics of electric current measured in the point of Jian Yu、Jian liao inserted（Man=m、Woman=w）,N 性别 年龄（岁）电流变化（A）电流差值（A）峰值出现（次）Sex age（years）electr
5、ic current electric current difference peak value 1 w 69-2013 33 42 m 52-943 52 53 w 65-4418 62 54 m 78-3716 53 6 5 w 75-423 27 46 w 77-667 73 47 m 72-2448 72 58 w 54-1239 51 59 m 23-2547 72 6,实验1-2 动物针刺电化学电流研究,方法 将12只ICR实验小鼠按其存活及处死（强力颈椎脱位法）后的整个时间过程分为：A组（健康活体），B组（死亡1 h），及C组（死亡28 h）；先将针灸针分别刺入每只经麻醉的IC
6、R实验小鼠左侧的曲池与后三里穴，用数字显示电流仪测量观察2min并记录产生的电流变化，后将该小鼠处死；待该小鼠处死1 h、28 h后再分别对其右侧的曲池与后三里穴，并用数字显示电流仪测量观察2min并记录产生的电流变化；记录的数据再制表分析。,结果 针刺实验小鼠A组的所发电流在83 1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流的平均差值为35.92A；B组所发电流在41-1A区间，并一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为21.58A；C组所发电流在148 1A区间，一直呈现由高向低的电流变化，电流发生率为100，电流平均差值为68.92A。,表1-2
7、、12只小鼠活时及死后针刺曲池、足三里穴所发电流发生率分析表（A）,No 体重g A组 A组差值 B组 B组差值 C组 C组差值1 19.4 286 22 2402412037 832 18.5 214 17 2332010243 593 19.1 362 34 9-11012367 564 21.2 369 27 34232438 355 18.3 152 13 167095913 466 20.0 832 81 383359618 787 18.3 588 50 416353812 268 19.7 344 30 3113014840 1089 17.9 618 53 122101121
8、11110 20.0 591 58 24222893 8611 17.3 233 20 1101110428 7612 18.5 282 26 265219330 63合计 228.2 831 431 41-1 259148 1 827均数 19.02 35.92 21.58 68.92发生率 100 100100,分析 证明该实验所测电流为针刺电化学效应的依据如下：,Analysis The current experiment provides for the evidence on the effects of acupuncture electrochemical.,A、由于无皮肤的组
9、织电阻为300800，因此实验1-1所测到的-44 67A瞬间电流变化区间的电流差值为27 73A。而其瞬间电压变化区间（根据欧姆定律）应为：8.1V58.4V，明显远远大于50100mV的细胞动作电位；因此该瞬间电流不是我们已知的生物内在电流。而实验1-2所测的83 1A、41-1A、148 1A瞬间电流变化区间的平均电流差值为35.92A、21.58A、21.58A，并一直呈现由高向低的电流变化，电流发生率为100，应与上述实验1-1的结果相同。,A、The organization skin resistance was 300 to 800 cm on without skin,so
10、the margin of transient current changes was 27 73 A on the interval-44 67 A.While the instantaneous voltage(according to Ohms law)was 8.1 V 58.4V,obviously far greater than the 50 100 mV of action potentials；As such,the current was not biological internal currents as we know。,B、此外，笔者曾在2002年的离体动植物（猪肉
11、、草莓）电化学测定实验中也明显证实了（结果与此次活体实验一样）其间也能产生uA级的电流。张大同.不同材质针灸针的电化学实验，上海针灸杂志，2003，22（5）：33-34.,B、In addition，the result also was proved by in vitro electrochemical plants and animals of the experiment in 2002(with the same vivo experiments)which could also have a Class A A Electricity.张大同.不同材质针灸针的电化学实验.上海针灸
12、杂志,2003.22（5）:33-34.,C、电化学科学规律。,C、The existing electrochemical universal laws of science.,实验1 结论 针刺穴位组织（能改变电位差）具有电化学原电池效应。,Experiment 1 Conclusion Acupuncture points(to change the potential difference)have the original battery electrochemical effect.,实验2 针行方便揿针的腐蚀研究,Experiment 2 Clinical studies of
13、acupuncture needles corrosion（The control group did not join because of original limited to the length,it was added as follows）,方法 将3600枚方便揿针针随机分成实验组（3000枚）和对照组（600枚），在一年中，实验组的3000枚方便揿针在临床反复灭菌使用，对照组600枚方便揿针也随实验组一起反复灭菌但不使用，其间由目测挑选出具有可见腐蚀斑的方便揿针（数据采用x2检验处理，P0.05有统计学意义），再选典型腐蚀的方便揿针用电子显微镜放大拍摄分析。,Methods
14、3,600 acupuncture needles were divided into the experimental group(3000)and the control group(600)randomly.3000 acupuncture needles were used repeatedly by sterilization,and 600 acupuncture needles were sterilized again and again as the experimental group but without using，too.Meantime,selected corr
15、osion spot with the acupuncture needle by the visual visible(data processing using x2 test,P 0.05 was significantly),the re-election of typical corrosion acupuncture needles to be amplified with electronic microscope.,结果 目测出实验组腐蚀针259枚（电子显微镜：腐蚀方便揿针表面呈现部分较严重腐蚀缺损区域），而对照组目测腐蚀针为0枚；实验组与对照组比较，P0.05差异有显著意义。
16、请见具体资料。,Result 259 needles were corroded in the experimental group(electron microscope:acupuncture needle surface corrosion showed some of the more serious corrosion defect regions),while 0 needle was corroded in the control group by eyeballing;The result of experimental group compared with the cont
17、rol group,P 0.05 significant difference.See specific information.,表2、实验组方便揿针目测腐蚀率发生分析表Table 2、The experimental group stainless steel acupuncture needles visual analysis of corrosion rate in table,时间（月）腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率Time（A month）Corrosion pin counts No corrosion pin counts The total number of
18、 needle Corrosion percentage13 0 3000 3000 046 19 2981 3000 0.6379 51 2930 2981 1.711012 189 2741 2930 6.45Total 259 2741 3000 8.63,表3、实验组与对照组目测方便揿针腐蚀率的比较Table 3、The experimental group and control group visual stainless steel acupuncture needle corrosion rate comparison,腐蚀针数 未腐蚀针数 分析总针数 腐蚀百分率 Corros
19、ion pin counts No corrosion pin counts The total number of needle Corrosion percentage实验组 259（8.63）2741（91.37）3000 8.63(The experimental group)对照组 0（0）600（100）600 0(The control group)Total 259 3341 3600 7.19x 2=55.81562406 P0.05,方便揿针的电子显微镜照片所示腐蚀现象 见图1、2、3,Corrosion acupuncture needle shown in the el
20、ectron microscope photographs of corrosion phenomenonSee figure 1,2,3,图1 放大50倍的腐蚀方便揿针局部Figure 1 Large 50 times the local corrosion acupuncture needles,图2 放大100倍的腐蚀方便揿针局部Figure 2 Large 100 times the local corrosion acupuncture needles,图3 放大1000倍的腐蚀方便揿针局部Figure 3 Large 1000 times the local corrosion a
21、cupuncture needles,实验2 结论 针刺穴位组织会使针体腐蚀，该腐蚀现象符合电化学腐蚀原理。,Experiment 2 ConclusionThe point of acupuncture needles willcorrode the acupuncture needles,corrosion phenomenon is consistent with the principle of electrochemical corrosion.,实验3 针刺穴位组织铬、镍、铁元素含量变化的研究,Experiment 3 The study on the change of elem
22、ent of acupuncture Points with chromium,nickel,iron.,方法 将15只实验兔按A（方便揿针10枚刺入左环跳穴区电针仪通电留针5h）、B（方便揿针10枚刺入右臂臑穴区留针5h）实验组及C（右环跳穴区）、D（右臂臑穴区）对照组，进行实验，5h后对60个（每点15个）样本用(ICP-MS)电感耦合等离子体质谱仪（对与方便揿针主体相关联的铬、镍、铁元素的含量）进行分析，数据采用t检验处理，P0.05有统计学意义。,Methods 15 rabbits were divided into the experimental groups and contr
23、ol groups，the experimental groups were A(10 acupuncture needles piercing left Central Point district jump-electro-acupuncture needle telegram to stay 5 h)，and B(10 acupuncture needles piercing right arm HDL Points district to stay 5 h).The control groups were C(right ring jump Point),and D(right arm
24、 HDL Point district.60 samples(15 per point)were analyzed by electricity flu coupled plasma mass spectrometer(with stainless steel acupuncture needles linked to the main chromium,nickel,iron content),data processing using t-test,P 0.05 with statistical significance.,结果 实验组铬、镍、铁元素含量大幅升高，与对照组比较，P0.001
25、其差异具显著意义。见表4、表5。,Result The experimental group chromium,nickel,iron content significantly increased,compared with the control group,P 0.001 with its significant difference.See Table 4 and Table 5.,表4 两组臂臑穴区铬、镍、铁元素含量的比较（S，ng/g，各组n=15）Table 4、Compare of number of Cr、Ni、Fe in the point of Jian Yu betwe
26、en two groups,铬Cr 镍Ni 铁Fe实验组（留针）270.0168271.63 116.1608743.87 27060.80411332.91(stay needle)对照组Control 54.00709012.76 2.73888721.949 5523.45362380.729 Compared with the control group（与对照组比较）:P0.001,表5 两组环跳穴区铬、镍、铁元素含量的比较（S，ng/g，各组n=15）Table 5、Compared with the element of Cr、Ni、Fe in the point of Jian
27、 liao between two groups,铬Cr 镍Ni 铁Fe实验组（电针）303.93884115.7 134.7799164.55 14175.5915327.276(Electroacupuncture)对照组Control 58.01403019.39 5.31042624.824 5414.27642531.767Compared with the control group（与对照组比较）:P0.001,实验3 结论 针刺穴位组织（能够增加离子、微量元素）具有电化学原电池效应。,Experiment 2 ConclusionAcupuncture points(to in
28、crease ions,trace elements)have the original electrochemical battery effect.,总体结论 按上述4项实验所取得的证据及电化学、微观腐蚀原电池效应科学规律，所共同组成的证据锁链。笔者认为：针刺穴位组织的原始效应物质为电子、微量元素；针刺穴位组织具有电化学原电池（能够增加电子、离子、微量元素）效应。,The overall conclusion The study results show that the original materials of acupuncture points organization are e
29、lectronics,trace elements,and acupuncture points organization have the original electrochemical battery effect.(To increase electrons,ions,trace elements),according to the above three of evidences obtained by the experiment,electrochemical and corrosion original micro-battery effect scientific laws,
30、which together constitute the evidence chain.,上述实验课题针刺（含电针）电化学效应的相关实验及其意义2008年12月已经通过鉴定验收，并在省科技厅成果登记（成果登记号：09001242）。（鉴定、验收）评价：,一、该项目提供的技术资料齐全，符合验收要求。二、该研究通过数字显示电流仪测定针刺ICR实验小鼠活体、尸体穴位、人体穴位组织所发电流的定性、定量研究；通过可视的观察及电镜等观察临床使用针灸针具腐蚀现象的研究；通过ICP-MS电感耦合等离子体质谱分析仪对针刺实验兔穴位组织的相关微量元素含量变化的研究；取得以下成果：1、证明了方便揿针针刺最初的原始效应是建立在电化学效应基础上的穴区局部的电流、电位差变化；2、证明了针刺最初的原始效应物质是建立在电化学效应基础上的针刺穴区局部微量元素含量的改变（影响针刺穴区局部浓度差）所产生的效应；3、揭示了针刺效应机制是电化学效应。三、该课题思路新颖，设计合理，统计学方法得当，结果可靠。该研究填补了针刺电化学效应的研究空白，初步建立了针刺电化学效应的理论基础，对电化学效应在针灸机理及应用上具有较大的意义。该课题已撰写论文5篇。,
