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1、Structural confirmation of oligosaccharides newly isolated from suger beet molasses,Outlines,Background,Achievement,Experimental,Conclusion&Discussion,Background,Why should we do this experi-ment?raffinose;other oligosaccharides;examined in detail,What contributions could the study bring?new oligosa
2、ccharides;structure comfirmed;full assignment of the 1H,13C signals;2D-NMR,Four oligosaccharides were isolatedThe structural of them were confirmed,Achievement,1st,The first full assignment of 1H，13C and 2D-NMR of saccharide 14 were carried out,2nd,Experimental,Diluted five times with water,MOLASSES
3、,Preparative HPLCODS column35water3.5mL/min60 times,DILUTED MOLASSES,Measured with a Refractive Index DetectorConcentrated in vacuo then freeze-dried,ELUTEDSACCHARIDES,POWDERED FRACTIONS I to XII,Experimental,byHPAEC and TOF-MS analyses,Experimental,Experimental,1st,2nd,3rd,HPAECSaccharide14 were sh
4、own to be homogene-ous,but didnt correspond to any authentic saccharides,TOF-MSThe polymerization was established as 3,Acid HydrolyzatesHPLCSaccharides 1&2:Glucose,Fructose,Galactose(1:1:1)3&4:Glucose,Fructose(2:1),Methylation and methanolysis GLCMethylation was carried out by Hakomori methodMethano
5、lyzed by heating at 96 for 30min with 1.5%methanolic hydrochloric acidresult,saccharide 3,Methyl-1,3,4,6-tetra-O-methyl-D-fructoside,Methyl-2,3,4,6-tetra-O-methyl-D-glucoside,Methyl-2,3,4-tri-O-methyl-D-glucoside,-D-glucopyranosyl-(1-6)-D-glucopyranosyl-(1 2)-D-fructofuranoside,Experimental,Saccharide 3,Experimental,Your Text Here,Your Text Here,The arrangement of the sugar residues,Your Text Here,The anomeric proton(H-1)of Glc exhibited a correlation peak to the quaternary carbon(C-2)of Fru,Glc(1-6)to Glc(1 2”)to Fru,Experimental,Saccharide 3,Conclusion&Discussion,Thank You!,
