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1、Illumina 测序的原理和应用,Illumina测序流程,Adapter and Library 接头和文库,Illumina测序流程,什么是 flow cell？,Flow cell 即流动槽，如载玻片大小，有8个通道即8条lane。,1.Single DNA libraries are hybridized to primer lawn and extended by polymerase,2.Double-stranded molecule is denatured and original template is washed away,3.New synthesized stra
2、nd is covalently attached to flow cell surface to form a bridge and extended by polymerase4.Double-strand bridge is formed,5.Double-strand bridge is denatured and formtwo copies of covalently bound single-stranded templates6.Bridge amplification cycle repeat,Multiple bridges are formeddsDNA bridges
3、are denatured and reverse strands are cleaved,9.Cleaved reverse strands are washed away and leaving a cluster with forward strands only,Illumina测序流程,10.Sequencing primer is hybridized to adapter sequence,11.Only one nucleotide is added every cycle as the 3 end is blocked,12.Cleave the fluor and free the 3 end then repeat above steps for next cycle,13.Sequenced strand is stripped off and the index primer is added for to seq index,3端,5端,Index primer,14.Repeat bridge amplification to form reverse strand after index sequencing15.Sequencing the reverse strands,高通量测序的应用,高通量测序的应用,Thank you!,
