《《自噬检测方法》PPT课件.ppt》由会员分享，可在线阅读，更多相关《《自噬检测方法》PPT课件.ppt（9页珍藏版）》请在三一办公上搜索。
1、自噬检测方法,常用细胞器标记物,自噬体检测：1.透射电镜2.免疫荧光观察LC3斑点,（P62辅助说明）；3.WB检测LC3+P62,并增加自噬降解抑制剂处理组。实验顺序：3 1,自噬流评价：1.双标LC3,红色斑点/黄色斑点;2.LC3与lamp1的共定位；3.DQ-BSA;4.稳转GFP-LC3细胞中检测游离GFP,Fusion of autophagosomes with endosomes/lysosomes is mediated by the class C VPS complex,Rab GTPase and the SNARE complex.,Cai,H.,Reinisch,K
2、.&Ferro-Novick,S.Coats,tethers,Rabs,and SNAREs work together to mediate the intracellular destination of a transport vesicle.Dev.Cell 12,671682(2007).Itakura,E.,Kishi-Itakura,C.&Mizushima,N.The hairpin-type tail-anchored SNARE syntaxin 17 targets to autophagosomes for fusion with endosomes/lysosomes.Cell 151,12561269(2012).Takts,S.et al.Autophagosomal Syntaxin17-dependent lysosomal degradation maintains neuronal function in Drosophila.J.Cell Biol.201,531539(2013),机制研究：CO-IP比较各组处理后stx17结合的SNAP29和vamp8,
