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1、测序通用引物载体名称 P3*FLAG-CMV pAc5.1/V5-His(_A,_B,_C) pAcG2T pACT pACT2 pACT2 pACYC184(BamHI-site) pACYCDuet-1(MCSI) pACYCDuet-1(MCSII) pAdEasy-1 pAdTrack-CMV pAS2-1 pB42AD pBABE pBacPAK8 pBAD pBAD/HisABC pBBR1MCS pBD-GAL4 pBI121 pBIND pBK-CMV pBluescriptIIKS(+/-) pBluescriptIISK(+/-) pBluescriptKS(+/-) pB
2、luescriptSK(+/-) pBR322(BamHI-site) pBR322(EcoRI/HindIII-sites) pBR325(BamHI-site) pBR329(BamHI-site) pBridge(MCSI) pBridge(MCSII) pBS-T II pBS pBSR pBudCE4.1(MCSI) pBudCE4.1(MCSII) pBudCE4.1/lacZ/CAT pBV220 pCAL-c 上游引物 CMV-30(825-854) pAc5-5 pGEX-5 T7EEV GAL4 ADfor p17110 PBRforBam ACYCDuetUP1 Prim
3、er DuetUp2 not available not available GAL4 Bdfor/P24990 pB42ADF pBMN_5 BAC1 pBAD-F pBAD-5 T3/M13rev pBD-GAL4-F 35S GAL4-Bdfor M13F/T7 T3/M13rev T3/M13rev T3/M13rev T3/M13rev PBRforBam pBRforEco PBRforBam PBRforBam GAL4-Bdfor not available T3/M13rev T7 T3 CMV-Profor,T7 EF-1 Fwd CMV-Profor,T7 pBV220F
4、 T7/Tac-Profor 下游引物 CMV-24(1129-1152) BGH rev not available T3/EBVrev pGAL4-ADrv p12584 PBRrevBam DuetDOWN1 T7-Terminator not available not available pGAL4-BDrv/P31430 PB42ADR BAC2 pBAD-R T7/M13for PBD-GAL4-R NOS T3,EBVrev M13R/T3 T7/M13for T7/M13for T7/M13for T7/M13for PBRrevBam pBRrevHind PBRrevBa
5、m PBRrevBam 3BD not available T7/M13for T3 T7ter c-mycrev,EBVrev BGH c-mycrev,EBVrev PBV220R T7-Terminator pCAL-n pCAMBIA1301(1300) pCAMBIA1304 pCAMBIA2300 pCANTB5E pCAT3-enhancer pCDFDuet-1(MCSI) pCDFDuet-1(MCSII) pcDNA1.1 pcDNA2.1 pcDNA3 pcDNA3.1(+/-) pcDNA3.1/His(_A,_B,_C) pcDNA3.1/myc-His(_A,_B,
6、_C) pcDNA3.1/V5-His(_A,_B,_C) pcDNA3.1/Hygro(+,-) pcDNA3.1/Zeo(+.-) pcDNA3.3TOPOTA pcDNA4/HisMax(_A,_B,_C) pcDNA4/HisMax-TOPO pcDNA4/HisMax-TOPO/lacZ pcDNA4/myc-His(_A,_B,_C) pcDNA4/TO pcDNA4/V5-His(_A,_B,_C) pcDNA4-TET/ON/AMP+ pcDNA5/FRT pcDNA6/myc-His(_A,_B,_C) pcDNA6/V5-His(_A,_B,_C) pCEP4 pCI pC
7、I-neo pCMS-EGFP pCMS-EGFP pCMV-3Tag-4A pCMV5 pCMV6 pCMV6-XL4 pCMV-HA pCMV-MYC(AMP+) pCMVmyc/nuc pcmv-scriptEXVector pCMV-Sport6 pCOLADuet-1(MCSI) T7/Tac-Profor pCAMBIA1301-5(ECORI)/M13R pCAMBIA1301-5(ECORI) M13R(-48) S1 RVP3 ACYCDuetUP1 Primer DuetUP2 CMV-Profor,T7 M13for/T7 T7/CMV-Profor pcDNA3.1F/
8、T7/CMV-Profor T7/CMV-Profor T7/CMV-Profor T7/CMV-Profor T7/CMV-Profor T7 CMV-Profor Xpressfor Xpressfor Xpressfor T7,CMV-Profor TREfor/CMV-Profor CMV-Profor,T7 CMV-Profor T7/CMV-Profor CMV-Profor,T7 CMV-Profor,T7 pCEP-F/CMV-Profor T7 T7 T7 T7 T3 /PFLAG-CMV-F pCMV5F CMV-Profor CMV-Profor/T7 PCMV-F,CM
9、V-Profor,TREfor PCMV-F CMV-Profor T3 T7/M13for ACYCDuetUP1 Primer T7-Terminator pCAMBIA1301-3(HindIII) pCAMBIA1301-3(HindIII) M13F(-47)/M13F(-49) S6 此载体无反向引物 DuetDOWN1 T7TER pCDM8-3 M13rev/Tac-Profor BGH rev/SP6 BGH rev BGH rev BGH rev/c-mycrev BGH rev/V5rev BGH rev/V5rev BGHrev TKPAreverse BGH rev
10、BGH rev BGH rev BGH rev,c-mycrev BGH rev BGH rev,V5rev BGH rev BGH rev,c-mycrev BGH rev,V5rev EBVrev EBVrev EBVrev,T3 T3 EBVrev,T3 T7 PCMV5R pCMV6-3、HGHrev pCMV6-3、HGHrev PCMV-R，EBVrev PCMV-R, EBVrev BGH rev,c-mycrev T7 SP6/M13rev DuetDOWN1 pCOLADuet-1(MCSII) pCR2.1 pCR2.1-TOPO pCR3.1 pCR3.1-Uni pCR
11、4 pCR4-Blunt pCR4-TOPO pCR-Blunt pCR-BluntII pCR-BluntII-TOPO pCRII pCRII-TOPO pCR-ScriptSK(+) pCR-XL-TOPO pCS2+(MCSI) pCS2+(MCSII) pDB_GAL4cam pDB_leu pDEST22 pDEST15 pDEST20 pDEST26 pDisplay pDNR-LIB(MCS_A) pDonar pDONR201 pDONR207 pDONR223 pDrive pDsRED1-C1 pDsRed1-N1 pDsRed2-N1 pDsRed2-C1 pDsRED
12、2-C1(KAN+) pDsRed-Express pDSRED-monomer-N1(KAN+) pDSRED-N1(KAN+) pEASY-Blunt pEASY-BluntSimple pEASY-T1 Simple DuetUP2 T7,M13for M13for,T7 T7 T7 T3/M13rev T3/M13rev M13rev,T3 M13rev M13rev/SP6 M13rev/SP6 M13rev/SP6 M13rev/SP6 T7/M13for M13rev SP6 T3 T7P PDBLeu-5 DEST22F pGEX-5 pGEX-5 TREfor/CMV-Pro
13、for T7/CMV-Profor M13for,T7 M13F PDONR-F PDONR-F M13F T7/M13rev (DsRed1-N-f) CMV-Profor (DsRed1-N-f) (DsRed1-N-f) pDsRED-ex-C1-F dsRed1_N_primer/CMV-F dsRed1_N_primer pEGFP-N-5 M13F M13F M13F T7TER M13rev M13rev BGH rev BGH rev T7/M13for T7/M13for M13for,T7 T7,M13for T7/M13for M13for/T7 T7/M13for M1
14、3for/T7 T3/M13rev T7,M13for EBVrev,T7? pSG5-3 PDBLeu-3 DEST22R T7-Terminator pFastBac-3 T7/M13for c-mycrev pSG5-3,M13R M13R PDONR-R PDONR-R M13R SP6/M13for DsRed1-C-r RFP-Nrev,CMV-R? DsRed1-C-r DsRed1-C-r pEGFP-N-3 dsRed1_C_primer/CMV-R pDSRED-N-R T7/ M13R T7/ M13R M13R pEASY-T1 pEASY-T3 pECFP-N(C)
15、pEF/myc/cyto/ER/mito/nuc pEF1(4,6/myc-His) pEF1(4,6/V5-His) pEF1(4;6/His_A;_B;_C) pEF5/FRT/V5-D(-TOPO) pEGFP pEGFP-1 pEGFP-C pEGFP-C1 pEGFP-C2 pEGFP-C3 pEGFP-F pEGFP-N pEGFP-N1 pEGFP-N2 pEGFP-N3 pENTR pET-11(-a,-b,-c,-d) pET-12(-a,-b,-c) pET-14b pET-15b pET-16b pET-17b pET-17xb pET-19b pET-20b(+) pE
16、T-21(-a,-b,-c,-d)(+) pET-22b(+) pET-23(-a,-b,-c,-d)(+) pET-24(-a,-b,-c,-d)(+) pET-25b(+) pET-26b(+) pET-27b(+) pET-28a(-b,-c)(+) pET-29a(-b,-c)(+) pET-3(-a,-b,-c,-d) pET-30_Ek/LIC pET-30_Xa/LIC pET-30a(-b,-c)(+) pET-31b(+) M13F/T7 M13F/T7 PECFP-N-5. pEF-5 T7/pEF-5 T7/pEF-5 T7/Xpressfor T7/pEF-5 M13r
17、ev not available PEGFP-C-5 EGFP-Cfor EGFP-Cfor EGFP-Cfor EGFP-Cfor PEGFP-N-5 CMV-Profor CMV-Profor CMV-Profor M13F T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 T7 S.tag/T7 T7 T7 T7 S.tag/T7 not available M13R M13R/SP6 PECFP-N-3, Pcdna3.1R/BGH rev Pcdna3.1R/BGH rev Pcdna3.1R/BGH rev Pcdna3.1R/BGH
18、rev Pcdna3.1R/BGH rev EGFP-Nrev EGFP-Nrev/ PEGFP-N-3 PEGFP-C-3 SV40-pArev SV40-pArev SV40-pArev SV40-pArev PEGFP-N-3 EGFP-Nrev EGFP-Nrev EGFP-Nrev M13R T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Termi
19、nator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator pET-32_Ek/LIC pET-32_XA/LIC pET-32a(-b,-c)(+) pET-33b(+) pET-34b(+) pET-35b(+) pET-36b(+) pET-37b(+) pET-38b(+) pET-39b(+) pET
20、-40b(+) pET-41_Ek/LIC pET-41a(-b,-c)(+) pET-42a(-b,-c)(+) pET-43.1a(-b,-c)(+) pET-43.1a_EK/LIC pET-44a(-b,-c)(+) pET-44a_EK/LIC pET-45b(+) pET-46_Ek/LIC pET-5(-a,-b,-c) pET-52b(+) pET-7 pET-9(-a,-b,-c,-d) pETcoco-1 pETDuet-1(MCSI) pETDuet-1(MCSII) pET-T4 pEYFP-C1 pEYFP-N1 pFLAG-ATS pFLAG-CTC pFLAG1
21、pFLAG-CMV(-2) pFLAG-CMV1 pGAD424 pGADGH pGADT7 pGADT7-Rec pGADT-T pGAD10 pGAPZa-A pGBKT7 TRXfor TRXfor S.tag/TRXfor T7 S.tag S.tag S.tag S.tag not available S.tag S.tag GST-Cfor S.tag/pGEX-5 S.tag/pGEX-5 S.tag S.tag S.tag S.tag T7 T7 T7 T7 T7 T7 T7 pETUP1 DuetUp2 T7 pEGFP-C-5 pEGFP-N-5,CMV-Profor N2
22、6for N26for N26for CMV-30；CMV-Profor CMV-30 GAL4_ADfor GAL4_ADfor T7/GAL4_ADfor T7/GAL4_ADfor T7 GAL4_ADfor a-FACTOR T7 T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminato
23、r T7-Terminator coliDOWN not available T7-Terminator not available T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator T7-Terminator DuetDOWN1 T7-Terminator T7-Terminator pEGFP-C-3 pEGFP-N-3 pTrcHis-rv；C24rev pTrcHis-rv；C24rev pTrcHis-rv；C24rev CMV-24 CMV-24 3AD 3AD 3
24、AD 3AD 3,BD 3AD 3AOX 3,BD pGBT9 pGEM-1 pGEM-3 pGEM-3Zf(+)(-) pGEM-4 pGEM-4Z pGEM-5Zf(+)(-) pGEM-7Zf(+)(-) pGEM-9Zf(+)(-) pGEM-T pGEM-T_Easy pGene/V5-His_A(_B,_C) pGEX-2T pGEX-2TK pGEX-3X pGEX-4T-1(-2,-3) pGEX-5X-1(-2,-3) pGEX-6P-1(-2,-3) pGL2 pGL3 pGL3-Basic(MCSI) pGL3-Basic(MCSII) pGL4 pGlow(-TOPO)
25、 pGM-T pGWC pHook-2 pIND/V5-His_A(_B,_C) PinpointTMVector pIRES(MCSI) pIRES(MCSII) pIRES2-EGFP pIRES-hrGFP-2a pIRESneo2 pIVEX(all) pLEXA pLNCX pLNCX2 pLVTHM pLXSN pMALc MATCHMAKER5DNA-BD/GAL4BD T7 T7 SP6/M13rev T7 T7/M13for SP6/M13rev SP6/M13rev T7/M13for T7/M13for T7/M13for pGENE-5 pGEX-5 pGEX-5 pG
26、EX-5 pGEX-5 pGEX-5 pGEX-5 GLP1 RVP3 RVP3(PGL3+)? RVP4, T7 T7 SP6 T7/CMV-Profor not available PinpointPrimer T7 not available CMV-Profor,pIRES2-EGFP.P5 CMV-Profor,T3 T7/CMV-Profor T7/pBRrevBam pLEXA-F pLNCX-F CMV-Profor H1primer pLXSN-F MalE primer MATCHMAKER3DNA-BD SP6 SP6 T7/M13for SP6 SP6/M13rev T
27、7/M13for T7/M13for SP6/M13rev SP6/M13rev SP6/M13rev BGH rev,V5rev pGEX-3 pGEX-3 pGEX-3 pGEX-3 pGEX-3 pGEX-3 GLP2 GLP2(RVP4)(没有特别说明用GLP2) GLP2(PGL3-)? PLXSN-3? pGlow-3 SP6 T7 not available BGH rev,V5rev SP6 pIRES-3 T3,EBVrev IRESrev,pIRES2-EGFP.P3 pIRES-hrGFP-3 pIRES-3 T7-Terminator pLEXA-R pLNCX-R p
28、RevTRE-3 SP6 pLXSN-R M13F(-47) pMAL-c2E pMAL-C2x pMALd pMALe pMAL-P4X pMAL-p2X pMET A/B/C pMD18-T pMD19-T pMD20-T pMOSBlue pMSCV-puro pMT/V5-His_A(_B,_C) pNTAP pCTAP pOTB7 pOptiVEC-TOPO TA pPC86 pPC97 pPIC3.5K pPic9k(A+,Z+) pPICZa pPROEXHTA pProEX-HTa(b,c) pPD129.36 pQE30or40or60(AMP+) pRC/CMV2/CAT
29、pRECEIVER pRECEIVER-M03 pREP4 pRK5 pRSET pRSFDuet-1(MCSI) pRSFDuet-1(MCSII) pSecTac2 pSG5 pSHlox1 pSHUTTLE-CMV pSI pSilencer1.0-U6 pSilencer2.0-U6 pSilencer2.1-U6neo pSilencer3.1-H1hygro MalE primer Pmal-c2x-F(P5) MalE primer MalE primer MalE primer MalE primer AUG1F M13R(-48)ECORI M13F47 M13F47 T7/
30、M13rev pLXSNfw Mtfor T3 pCTAP5primer M13rev/T7 CMV-F/CMV-Profor PPC86-F PBD-GAL4-F 5AOX 5AOX(Ppic9k-f)/a-factor 5AOX/PPICZa-F M13R(-48) M13R48/ Tac-Profor PQE30-F CMV/T7 CMV-F 此载体 pRECEIVER-M03F pREP4 forward primer SP6 T7/Xpressfor DuetUP1 DuetUP2 T7/CMV-Profor pSG5-5,/T7 T7 PSHUTTLE-CMV-F T7 T7 T7
31、 T7 M13F(-47) M13F(-47) Pmal-C2X-R(P3 ) M13F(-47) M13F(-47) M13F(-47) M13F(-47) AUG1R M13F(-47)(HIND) M13R48 M13R48 M13for pMSCVrev BGH rev,V5rev T7 T7 M13for/SP6 EMCVIRES reverse PPC86-R PBD-GAL4-R 3AOX 3AOX(Ppic9k-R) 3AOX 无反向引物 pBADrv/pTrcHis-3 M13F20/M13F PQE-30R 无反向引物 pRECEIVER-M03R EBV reverse
32、primer pSG5-3 T7ter DuetDOWN1 T7TER BGH rev/c-mycrev PSG5-R(SV40) SP6 PSHUTTLE-CMV-R EBVrev T3 M13for M13for 3.0REV pSilencer4_1-CMVneo pSILENCEV2.0-U6 pSIneo Psit pSK01-T pSK-CMV(KAN+) pSOS pSP64 pSP64/65 pSP65 pSP70 pSP71 pSP72 pSP73 pSport1 pSPORT1 pSPT18 pSTBlue pSUPER.Basic pSUPER pSURE-T pSURE
33、-Tsimple pSuperCos pT3T7 pT7-7 pT7Blue pT7T3_18U pT7T3D-PAC pTA2 pT-Adv pTAg pTARGETTM pTG19-T pTARGETTM pThioHisA,B,andC pTO-T7 pTRC99A pTrc99a-c pTRC99a-c pTrcHisB pTrcHisA pT-REX-DEST30 pTriEx1 pSilener4.1-5 T7 T7 T7 M13F T7 Psos5 SP6 SP6 SP6 SP6 SP6 SP6 SP6 SP6/M13F(-47) SP6/M13for SP6 T7/M13rev
34、 T7/M13F T7 M13F M13F-47 pBRforEco M13rev/T3 T7 T7 M13rev/T7 M13rev/T7 M13F/T7(优先使用M13F） M13rev T7/M13rev pTarget.F(在T7前面）/T7 M13F(-47) T7/M13F Trx Forward T7 PTRC99A-F PTRC99C-F PTRC99C-F PTrcHisA-F/Xpressfor PTrcHisA-F TREfor T7/TriExup CMV-Profor 2.0REV EBVrev,T3 T7T M13R T3 Psos3 M13rev 此载体无反向引物
35、 M13rev T7 T7 T7 T7 T7/M13R T7/M13rev T7 SP6/M13for M13R T3 M13R M13R-48 T7 M13for/T7 not available M13F(-47) M13for/T3 M13for/T3 T3/M13R T7,M13for M13for pTarget.R M13R(-48) PTARGET-SEQ Trx Reverse / M13for T7TER pTrcHis-rv PTRC99C-R PTRC99C-R PTrcHisA-R PTrcHisA-R T7 TriExdown pTriEx1.1 pTriEx1.1_
36、Hygro pTriEx1.1_Neo pTriplEX1 pTriplEX2 pTWIN-1 pTXB1 pTXB2 pTXB3 pTYB1 pTYB2 pTYB3 pTYB4 pTZ_18U pUB6/V5-His pUC13 pUC18 pUC18(19)/118(119) pUC19 pUC57 pUC8 pUC9 pUM-T pUCm-T(AMP+) pUNI/V5-His pVAX1T7 pVP22/mycHis pVP22/mycHis2 pWE15 pYES2 pYES-DEST52 pYEX_4T_1 pYEX_4T_2 pYEX_4T_3 pYEX_4T_4 pZeoSV2
37、+/hu-Tyr pZERO-1 pZERO-2 pZome-1-C pZome-1-N YCp50(BamHI-site) YEp13(BamHI-site) YEp24(BamHI-site) T7/TriExup T7/TriExup T7/TriExup TriplEx-LD-5 TriplEx-LD-5；pTR5 T7 T7 T7 T7 T7 T7 T7 T7 T7/M13rev UB-F M13rev/M13rev(-48) M13rev/M13rev(-48) M13F(-47) M13rev/M13rev(-48) M13rev/M13rev(-48) M13rev/M13re
38、v(-48) M13rev/M13rev(-48) M13F(-20) M13F/T7 not available T7/CMV-Profor VP22-Cfor T7/CMV-Profor pBRrevHind T7 T7/GAL1-Profor Clontech pGEX-5 pGEX-5 pGEX-5 T7 M13rev/SP6 M13rev/SP6 Zome-F Zome-F PBRforBam PBRforBam PBRforBam TriExdown not available IRESrev T7,TriplEx-LD-3 T7,TriplEx-LD-3 T7TER MxeInt
39、einrev MxeInteinrev MxeInteinrev Inteinrev Inteinrev Inteinrev Inteinrev M13for BGH rev,V5rev M13for/M13for(-49) M13for/M13for(-49) M13R(-48)/PUC19(HIND) M13for/M13for(-49) M13for/M13for(-49) M13for/M13for(-49) M13for/M13for(-49) M13R(-20) M13R BGH rev,V5rev PCDNA3.1R/BGHR BGH rev,c-mycrev VP22-Nrev T7 pYes2.R V5rev/CYC1-Terminator pYEX_4T-3 pYEX_4T-4 pYEX_4T-4 pYEX_4T-4 not available T7/M13for M13for/T7 Zome-R Zome-R PBRrevBam PBRrevBam PBRrevBam YIp5(BamHI-site) RNAi-ReadypSIREN-RetroQ-U6ZsGreen 菌PCDB 天为时代TA载体 m13 pMSCV-neo PBRforBam U6 T7 T7 T7？ pMSCV-5 PBRrevBam BGH T3 T3？ pMSCV-3
