《论文（设计）基于碳纳米管结构的阻抗分析检测癌症细胞24688.doc》由会员分享，可在线阅读，更多相关《论文（设计）基于碳纳米管结构的阻抗分析检测癌症细胞24688.doc（3页珍藏版）》请在三一办公上搜索。
1、基于碳纳米管结构的阻抗分析检测癌症细胞刘洋，刘绍琴*微系统与微结构制造教育部重点实验室，哈尔滨工业大学，哈尔滨，150080*Email: shaoqinliu, shaoqinliu从实体瘤分离的循环肿瘤细胞（CTCs）进入血液系统，可以在体内的不同组织中发生转移1。虽然早期癌症病人CTCs的数量极少，但是CTCs的精确检测已被证明对病情的监督和预测有着重要的意义2。然而，外周血中CTCs的含量很少，因此检测方法需要高的灵敏度和特异性，这就对CTCs 的临床检测提出了巨大的挑战3。因此我们提出了一种快速简单阻抗分析法用于检测CTCs。该传感装置基于组装在ITO电极表面的碳纳米管（CNTs）结
2、构，检测机理是肿瘤细胞与电极表面的上皮细胞粘附分子抗体（anti-EpCAM）的特异性结合。CTCs的结合会使传感器阻值发生改变，并且阻值变化与细胞浓度的对数成线性关系，线性范围为10-105个细胞/毫升。我们所提出的阻抗分析传感装置可以灵敏地检测全血中的肿瘤细胞，并且无需样品的预前处理步骤。因此该传感器可能成为定点照护医疗诊断中一种快速，灵敏，经济的方法。关键词：循环肿瘤细胞（CTCs）；电化学阻抗传感器；碳纳米管（CNTs）参考文献1 Escosura-Muniz, A.; Sanchez-Espinel, C.; Diaz-Freitas, B. Anal Chem. 2009,81(2
3、4):10268.2 Huang, Y.; Cai, D.; Chen, P. Anal Chem. 2011, 83(12):4393.3 Wang, S. T.; Wang, H.; Jiao, J. Angew Chem Int. 2009, 48(47):8970.Carbon Nanotube Architectures for the Impedimetric Selective Detection of Cancer CellsYang Liu, Shaoqin Liu*Key Laboratory of Microsystems and Microstructures Manu
4、facturing, Ministry of Education, Harbin Institute of Technology, Harbin, 150080Circulating Tumor Cells (CTCs) detach from the solid primary tumor into the bloodstream and may travel to different tissues of the body giving rise to metastasis. Although CTCs are very rare in the early stage of cancer
5、patients, precise detection of CTCs has been proven to significantly aid supervisory control of the patients condition and increase the possibility of positive prognosis. However, owing to the rarity of CTCs in peripheral blood, detecting these cells requires methods combined with high sensitivity a
6、nd specificity, which sets tremendous challenges for the implementation of these assays into clinical routine. Herein we present a fast and simple impedimetric sensing approach for detection of CTCs. The sensing device is based on a nanostructured architecture of carbon nanotubes (CNTs), which are a
7、ssembled on ITO electrode surface. The detection mechanism relies on the specific affinity between tumor cells and epithelial-cell adhesion molecule antibody (anti-EpCAM) anchored on the modified electrode surface. The binding of tumor cells to anti-EpCAM causes the change of the resistance and the
8、increase of resistance values was log-linear in the range of 10 to 105 cells per ml. The proposed impedimetric sensing device allows for the sensitive detection of cancer cells from whole-blood samples without any further sample preparation steps. Thus, the proposed sensor has the potential to becom
9、e a rapid, sensitive, and cost-effective assay for point-of-care diagnostics. Editors note: Judson Jones is a meteorologist, journalist and photographer. He has freelanced with CNN for four years, covering severe weather from tornadoes to typhoons. Follow him on Twitter: jnjonesjr (CNN) - I will alw
10、ays wonder what it was like to huddle around a shortwave radio and through the crackling static from space hear the faint beeps of the worlds first satellite - Sputnik. I also missed watching Neil Armstrong step foot on the moon and the first space shuttle take off for the stars. Those events were w
11、ay before my time.As a kid, I was fascinated with what goes on in the sky, and when NASA pulled the plug on the shuttle program I was heartbroken. Yet the privatized space race has renewed my childhood dreams to reach for the stars.As a meteorologist, Ive still seen many important weather and space
12、events, but right now, if you were sitting next to me, youd hear my foot tapping rapidly under my desk. Im anxious for the next one: a space capsule hanging from a crane in the New Mexico desert.Its like the set for a George Lucas movie floating to the edge of space.You and I will have the chance to
13、 watch a man take a leap into an unimaginable free fall from the edge of space - live.The (lack of) air up there Watch man jump from 96,000 feet Tuesday, I sat at work glued to the live stream of the Red Bull Stratos Mission. I watched the balloons positioned at different altitudes in the sky to tes
14、t the winds, knowing that if they would just line up in a vertical straight line we would be go for launch.I feel this mission was created for me because I am also a journalist and a photographer, but above all I live for taking a leap of faith - the feeling of pushing the envelope into uncharted te
15、rritory.待添加的隐藏文字内容1The guy who is going to do this, Felix Baumgartner, must have that same feeling, at a level I will never reach. However, it did not stop me from feeling his pain when a gust of swirling wind kicked up and twisted the partially filled balloon that would take him to the upper end of
16、 our atmosphere. As soon as the 40-acre balloon, with skin no thicker than a dry cleaning bag, scraped the ground I knew it was over.How claustrophobia almost grounded supersonic skydiverWith each twist, you could see the wrinkles of disappointment on the face of the current record holder and capcom
17、 (capsule communications), Col. Joe Kittinger. He hung his head low in mission control as he told Baumgartner the disappointing news: Mission aborted.The supersonic descent could happen as early as Sunday.The weather plays an important role in this mission. Starting at the ground, conditions have to
18、 be very calm - winds less than 2 mph, with no precipitation or humidity and limited cloud cover. The balloon, with capsule attached, will move through the lower level of the atmosphere (the troposphere) where our day-to-day weather lives. It will climb higher than the tip of Mount Everest (5.5 mile
19、s/8.85 kilometers), drifting even higher than the cruising altitude of commercial airliners (5.6 miles/9.17 kilometers) and into the stratosphere. As he crosses the boundary layer (called the tropopause), he can expect a lot of turbulence.The balloon will slowly drift to the edge of space at 120,000
20、 feet (22.7 miles/36.53 kilometers). Here, Fearless Felix will unclip. He will roll back the door.Then, I would assume, he will slowly step out onto something resembling an Olympic diving platform.Below, the Earth becomes the concrete bottom of a swimming pool that he wants to land on, but not too h
21、ard. Still, hell be traveling fast, so despite the distance, it will not be like diving into the deep end of a pool. It will be like he is diving into the shallow end.Skydiver preps for the big jumpWhen he jumps, he is expected to reach the speed of sound - 690 mph (1,110 kph) - in less than 40 seco
22、nds. Like hitting the top of the water, he will begin to slow as he approaches the more dense air closer to Earth. But this will not be enough to stop him completely.If he goes too fast or spins out of control, he has a stabilization parachute that can be deployed to slow him down. His team hopes it
23、s not needed. Instead, he plans to deploy his 270-square-foot (25-square-meter) main chute at an altitude of around 5,000 feet (1,524 meters).In order to deploy this chute successfully, he will have to slow to 172 mph (277 kph). He will have a reserve parachute that will open automatically if he los
24、es consciousness at mach speeds.Even if everything goes as planned, it wont. Baumgartner still will free fall at a speed that would cause you and me to pass out, and no parachute is guaranteed to work higher than 25,000 feet (7,620 meters).It might not be the moon, but Kittinger free fell from 102,800 feet in 1960 - at the dawn of an infamous space race that captured the hearts of many. Baumgartner will attempt to break that record, a feat that boggles the mind. This is one of those monumental moments I will always remember, because there is no way Id miss this.
