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1、,药物靶标发现与筛选基因靶标核糖核酸靶标蛋白靶标,、基因靶标1、在基因数据库中搜寻药物靶标(1)表达序列标志(expressed sequencetag,EST数据库(2)归纳逻辑设计程序,EST是从一个随机选择的cDNA克隆进行5端和3端单次测序获得的短的cDNA部分序列,代表一个完整基因的一小部分,在数据库中其长度一般从20到7000bp不等,平均长度为360120bp。EST来源于一定环境下个组织总mRNA所构建的cDNA文库,因此EST也能说明该组织中各基因的表达水平。首先从样品组织中提取mRNA,在逆转录酶的作用下用o1igo(dT)作为引物进行RT-PCR合成cDNA,再选择合适的
2、载体构建cDNA文库,对各菌株加以整理,将每个菌株的插入片段根据载体多克隆位点设计引物进行两端一次性自动化测序,这就是EST序列的产生过程。,Quantitative real-time RT-PCR of upregulated or downregulated genes randomlyselected from the libraries of human normal SN(f)and PDs SNA的的Iav)a)GN(A$Kim,J.M.et al.DNA Res 2006 13:275-286;doi:10.1093/dnares/dsl016Copyright restric
3、tior apply.DNA RESEARCH,Immunohistochemical staining for th and alpha-tubulin in the sn of an MPTP micemodel(A)(a)(B)(a)alpha tubulinKim,J,M.eta.DNA Res200613:275286:doi:10.1093/dCopyright restrictions may apply.DNA RESEARCH,Cell death activity of differentially expressed genes in PD(A)Upregulated g
4、enessDownregulated genesCopyright restrictions may applyDNA RESEARCH,Amino Acid Sequences of Orexins(食欲素)and Orexin Receptors(A)Structures of mature orexin-A and-B peptidesC三“三圈翻圈噩圍籍I翻翻翻昌噩目l1器l=cell1998;92(4):573-585,Tissue Distribution of Rat prepro-orexin and Orexin Receptor mRNASAOrexinX2RB-actince1998;92(4):573-585,Localization ofprepro-orexin mRNAand immunoreactivePeptide in Adult Rat Braincell1998;92(4):573-585,Up-Regulation of prepro-orexin mRNA in Fasted RatsA霉33罪BgP0.0t糖 orexincell1998;92(4):573-585,
