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1、其他保存方法Other Preservation Methods,黃顯宗東吳大學M202,第三部份：食品保存與微生物,教學目標,認識其他食品保存方法與微生物之關係認識發展中的食品保存方法與微生物之關係,ASEPSIS,避免微生物污染食物的天然阻隔如 組織、表皮、硬殼等加工的無菌操作包裝處理,Removal of Microorganisms,Filtrationjuice,soft drink,beer,wine,waterCentrifugationSedimentationWashing,High Pressure Processing,Shape,color,and nutrients
2、of most foods are not affected.Commercially available in Japan since the early 1990s.Reference,Change in inactivation of Zygosaccharomyces bailii with pressure,http:/www.wsu.edu/gmhyde/433_web_pages/433Thermal-nontherm-procesg/HighHydroPressure.html,1個標準大氣壓等於101,325 Pa0.101325 MPa,Control of Listeri
3、a in meat by high pressure,http:/www.amif.org/AMIFResearch/1AMIpressure.pdf#search=High%20Pressure%20listeria,D value又出現了,Response of Bacteria,Mutants resistant to high pressure were foundStress response system stimulated,Pulsed Electric Fields,PEF,短暫高電壓的處理G較 G-耐性強，更不容易殺死孢子比營養細胞耐性強Stationary phase比
4、log phase 耐性強傷害細胞膜致細胞死亡與電的強度、處理時間、處理溫度有關,Electrolyzed Oxidizing Water,是利用稀鹽水加以電解，在陽極產生具pH值在26之間、氧化還原電位(oxidation reduction potential,ORP)為+1,000 mV以上，含有效氯並具有殺菌特性之酸性電解水酸性電解水，具有殺菌能力,Electrolyzed water,http:/.au/e-water/electrolyzed-water/how-does-it-work/,電解水處理 Listeria 菌,Liu and Su,2006,處理 E.coli,Sal
5、monella和 Listeria 菌,Venkitanarayanan et al.,1999,Manothermosonication,或稱為Thermoultrasonication同時結合熱與超音波處理Effective in reducing the thermal resistance of enzymes peroxidase,lipoxygenase and polyphenol oxidase.,Alternative Food Processing Technologies,Pulsed Light,Oscillating Magnetic Fields,Ultrasoun
6、d,X-ray.FDA http:/vm.cfsan.fda.gov/%7Ecomm/ift-toc.html,PEF處理Listeria菌,Inactivation kinetics of L.innocua NCTC 11289 at different electric field strengths.Symbols:,2.0 to 2.1 V/m;,2.3 to 2.5 V/m;,2.7 to 3.0 V/m;,3.2 to 3.5 V/m;,3.6 to 3.9 V/m.The pH of the treatment medium was 5,the conductivity was
7、 0.51 S/m at an inlet temperature of 40C,and the pulse length was 3.9 s.The inoculum contained 1.4 106 CFU/ml.The results are the means of data from two experiments,and standard deviations are indicated by error bars.,Wouters et al.,1999,使用Phages,如針對Listeria,E.coli,Salmonella等參考文獻,細菌形成，如乳酸菌Protein性質
8、,destroyed by digestive enzymesNontoxicHeat stable,storage stabilityNo off-flavors or off-odorsNarrow spectrum of antimicrobial activity舉例NisinSubtilin,against G+bacteria,like Nisin菌種改良,Bacteriocins,Bacteriocins-Nisin,http:/ans-bv.nl/Admin/mkstats/usage_200310.html,Bacteriocins,Nisin,Subtilin,Tetracyclines,Natamycin,
